Receptors for ceruloplasmin and ceruloplasmin copper in plasma membranes of rat heart and brain

Journal Article · Thu May 01 04:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:7025366

Goode, C A; Orena, S O; Linder, M C

Receptors for ceruloplasmin and its copper were investigated, using membrane preparations from rat heart and brain enriched in plasma membrane, as determined by the activity of 5'-nucleotidase. Specific binding was measured with fresh /sup 67/Cu-labeled ceruloplasmin, in the presence and absence of non-radioactive ceruloplasmin and/or Cu(II) (as the nitrilotriacetate complex; Cu-NTA). A 100-150-fold molar excess of non-radioactive ceruloplasmin and a 3300-4000-fold molar excess of non-radioactive Cu-NTA each reduced total binding of /sup 67/Cu-ceruloplasmin to about half. Inhibition of binding in the presence of both non-radioactive ligands was approximately additive. Binding in the presence of excess Cu-NTA showed positive cooperativity and was half maximal at a concentration of 10/sup -7/M. The results suggest that binding of ceruloplasmin copper to cell membranes is a two step process, involving binding of the protein moiety to a specific receptor on the cell surface and subsequent transfer of the ceruloplasmin copper to an adjacent (copper) receptor.

• Research Organization: California State Univ., Fullerton
• OSTI ID: 7025366
• Report Number(s): CONF-8606151-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:6; ISSN FEPRA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BODY
BRAIN
CARDIOVASCULAR SYSTEM
CELL CONSTITUENTS
CELL MEMBRANES
CENTRAL NERVOUS SYSTEM
CERULOPLASMIN
COMPLEXES
COPPER
COPPER 67
COPPER COMPLEXES
COPPER ISOTOPES
DAYS LIVING RADIOISOTOPES
ELEMENTS
ENZYME ACTIVITY
ENZYMES
ESTERASES
GLOBULINS
GLOBULINS-ALPHA
HEART
HYDROLASES
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGANDS
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
METALLOPROTEINS
METALS
NERVOUS SYSTEM
NUCLEI
NUCLEOTIDASES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHOSPHATASES
PROTEINS
RADIOISOTOPES
RATS
REACTION KINETICS
RECEPTORS
RODENTS
TRACER TECHNIQUES
TRANSITION ELEMENT COMPLEXES
TRANSITION ELEMENTS
VERTEBRATES