Differential expression of secretogranin II and chromogranin A genes in the female rat pituitary through sexual maturation and estrous cycle

Journal Article · Thu Feb 28 23:00:00 EST 1991 · Endocrinology; (USA)

DOI:https://doi.org/10.1210/endo-128-3-1374· OSTI ID:5858630

Anouar, Y; Duval, J ^[1]

---

1. C.U.R.A. 256, Rennes (France)

Secretogranin II (SgII) is a protein of pituitary secretory granules released by LHRH-stimulated gonadotrope cells. Estrogens and androgens are modulators of SgII release. Experiments were performed to determine the regulation of expression of the SgII gene in the female rat pituitary, during sexual maturation and according to the estrous cycle. Age- and cycle-related changes in SgII mRNA content were estimated through cytoplasmic slot blot; SgII content was determined by western blotting; maturation of the protein was controlled through (35S)sulfate labeling. Variations in chromogranin A (CgA), another protein of secretory granules, were analyzed in the same experimental conditions to assess the specificity of SgII regulation. The pituitary SgII concentration increased between days 7 and 21 (2.2-fold) and then declined to the initial 7-day-old value. Simultaneously, the CgA concentration went through a maximum between days 14 and 21 and then strongly dropped to barely detectable levels in the adult pituitary. The SgII mRNA concentration followed roughly the same pattern as the protein. Moreover, the sulfation level remained constant between days 14 and 60. These results demonstrated a regulatory mechanism operating, during sexual maturation, on the SgII gene and not on the protein processing or on storage/release steps. In the 4-day cycling females, the pituitary SgII mRNA and protein contents were the lowest during estrus. They then increased to their highest values in diestrus II. Moreover, the sulfation level of SgII was significantly higher during estrus than during any other stage. Due to its low content level, variations in pituitary CgA could not be demonstrated during the cycle.

• OSTI ID: 5858630
• Journal Information: Endocrinology; (USA), Journal Name: Endocrinology; (USA) Vol. 128:3; ISSN ENDOA; ISSN 0013-7227
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANDROGENS
ANDROSTANES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CELL DIFFERENTIATION
DAYS LIVING RADIOISOTOPES
ENDOCRINE GLANDS
ESTROGENS
ESTROUS CYCLE
EVEN-ODD NUCLEI
GENE REGULATION
GENES
GLANDS
HORMONES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MESSENGER-RNA
NUCLEI
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PITUITARY GLAND
PROTEINS
RADIOISOTOPES
RATS
RNA
RODENTS
STEROID HORMONES
STEROIDS
SULFUR 35
SULFUR ISOTOPES
TRACER TECHNIQUES
VERTEBRATES