Influence of natural and recombinant interferons on development of antiviral condition and activity of natural killers

Journal Article · Sat Mar 01 04:00:00 EST 1986 · Dokl. Biol. Sci. (Engl. Transl.); (United States)

OSTI ID:5856242

Kuznetsov, V P; Avdeev, G I; Vyadro, M M; Leikin, Yu D; Frolova, I S

For the purpose of a preliminary estimate of the therapeutic potential of domestic recombinant alpha/sub 2/-component of human leukocytic interferon (rl) in vitro tests, the authors studied its ability to induce development of antiviral condition in diploid culture of human embryo fibroblasts and to activate the cytolytic effect of natural killers in relation to tumor cells, of the K-562 leukemia line and cells of lung adenocarcinoma. The authors used a medicinal form of rL which was derived by expression of a reconstructed gene in Escherichia coli cells. Part of the tests were conducted with an analogous preparation synthesized using another producer, Pseudomonas sp). The biological effect of both preparations was the same. For comparison, a natural preparation was used in all tests: human leukocytic interferon for injection, II(le). The authors studied activity of natural killers in a fraction of mononuclears isolated from blood of essentially healthy donors and from cancer patients. Cells were incubated for 2 h with various concentrations of interferons, then combined in a ratio of 25-50:1 with target cells labeled with /sup 51/Cr. Cytotoxic reaction was conducted for 4 (4-CTR) or 18 h (18-CTR) at 37/sup 0/C. Natural killers could thus be divided into two subpopulations: killer (4-CTR) and cytotoxic (18-CTR) cells. In preliminary tests, both preparations possessed the ability to active natural killers. The effective concentration for rL was within the limits of 1000-2000 IU/ml, and 50-200 Iu/ml for Le. The data on activation of natural killers in 16 oncological patients (primarily with lung cancer), the authors established that both rL and Le induced activation of natural killers in the overwhelming majority of cases in relation to K-562 target cells and adenocarcinomas of the lung.

• Research Organization: N. F. Gamalei Scientific-Research Institute of Epidemiology and Microbiology, Moscow, USSR
• OSTI ID: 5856242
• Journal Information: Dokl. Biol. Sci. (Engl. Transl.); (United States), Journal Name: Dokl. Biol. Sci. (Engl. Transl.); (United States) Vol. 284:1-6; ISSN DKBSA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIMITOTIC DRUGS
BACTERIA
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARCINOMAS
CELL CULTURES
CELL KILLING
CHROMIUM 51
CHROMIUM ISOTOPES
CONNECTIVE TISSUE CELLS
DIPLOIDY
DISEASES
DNA
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
EMBRYOS
ESCHERICHIA COLI
EVEN-ODD NUCLEI
FIBROBLASTS
GROWTH FACTORS
HEMIC DISEASES
INTERFERON
INTERMEDIATE MASS NUCLEI
ISOTOPES
LABELLED COMPOUNDS
LABELLING
LEUKEMIA
LEUKOCYTES
LYMPHOKINES
MAMMALS
MAN
MATERIALS
METABOLIC ACTIVATION
MICROORGANISMS
MITOGENS
NEOPLASMS
NUCLEI
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PLOIDY
PRIMATES
PROTEINS
PSEUDOMONAS
RADIOASSAY
RADIOISOTOPES
RECOMBINANT DNA
RESPIRATORY TRACT CELLS
SOMATIC CELLS
SYNTHESIS
TUMOR CELLS
VERTEBRATES