Genotoxic effects of five polycyclic aromatic hydrocarbons in human and rat mammary epithelial cells

Journal Article · Mon Jan 01 04:00:00 EST 1990 · Environmental and Molecular Mutagenesis; (USA)

DOI:https://doi.org/10.1002/em.2850150204· OSTI ID:5848379

Mane, S S; Purnell, D M; Hsu, Ih-chang ^[1]

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1. Univ. of Maryland School of Medicine, Baltimore (USA)

Five polycyclic aromatic hydrocarbons (PAHs) of different carcinogenic activities were evaluated for their effects on DNA synthesis ({sup 3}HTdR labeling index (L.I.)) of rat and human mammary epithelial cells (MEC) and for their effects on chromosomes in MEC-mediated sister chromatid exchange (SCE) assays. When compared with DMSO-treated cells, exposures of rat MEC to the two most potent carcinogens, i.e., 7,12-dimethylbenz(a)anthracene (DMBA) and benzo(a)pyrene (B(a)P), resulted in a 45-62% reduction in the L.I. of rat MEC. Another carcinogen, 20-methylcholanthrene (MCA), produced a 35-48% reduction in L.I., while the noncarcinogenic PAHs, 1,2-benzanthracene (BA) and benzo(e)pyrene (B(e)P), showed no effect. Similarly, exposures of human MEC to DMBA and B(a)P resulted in a 50-90% depression in L.I. while BA was significantly less effective. When co-cultivated with Chinese hamster V-79 cells in the presence of PAH, both rat and human MEC can activate and release the active metabolites to induce SCE in V-79 cells. Comparing depression of L.I., SCE, and in vivo carcinogenicity for the 5 PAHs, SCE mediated by rat MEC is better correlated with carcinogenicity in rat than L.I. depression.

• OSTI ID: 5848379
• Journal Information: Environmental and Molecular Mutagenesis; (USA), Journal Name: Environmental and Molecular Mutagenesis; (USA) Vol. 15:2; ISSN EMMUE; ISSN 0893-6692
• Country of Publication: United States
• Language: English

Related Subjects

3-METHYLCHOLANTHRENE
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
ANTHRACENE
AROMATICS
BENZOPYRENE
BIOLOGICAL EFFECTS
BODY
CHROMOSOMAL ABERRATIONS
CONDENSED AROMATICS
CONNECTIVE TISSUE CELLS
DIMETHYLBENZANTHRACENE
DMSO
DNA REPLICATION
EPITHELIUM
FIBROBLASTS
GENETIC EFFECTS
GLANDS
HYDROCARBONS
HYDROGEN COMPOUNDS
MAMMALS
MAMMARY GLANDS
MUTATIONS
NUCLEIC ACID REPLICATION
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
POLYCYCLIC AROMATIC HYDROCARBONS
RATS
RODENTS
SISTER CHROMATID EXCHANGES
SOMATIC CELLS
SULFOXIDES
TISSUES
TRITIUM COMPOUNDS
VERTEBRATES