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Comparative cytogenetic analysis of bone marrow damage induced in male B6C3F1 mice by multiple exposures to gaseous 1,3-butadiene

Journal Article · · Environ. Mutagen.; (United States)
; ; ;

Groups of male B6C3F1 mice were exposed to ambient air or to gaseous 1,3-butadiene (BD) at 6.25, 62.5, and 625 ppm for 10 exposure days. Exposure to BD induced in bone marrow: 1) a significant increase in the frequency of chromosomal aberrations (CA); 2) a significant elevation in the frequency of sister chromatid exchanges (SCE); 3) a significant lengthening of the average generation time (AGT); 4) a significant depression in the mitotic index (MI): and, as measured in the peripheral blood, 5) a significant increase in the proportion of circulating polychromatic erythrocytes (% PCE), and 6) a significant increase in the level of micronucleated PCE (MN-PCE) and micronucleated normochromatic erythrocytes (MN-NCE). The most sensitive indicator of genotoxic damage was the frequency of SCE, followed by MN-PCE levels, and then by CA and MN-NCE frequencies. The most sensitive measure of cytotoxic damage was AGT followed by % PCE and then my MI. The extent of concordance ranged from a very good correlation between the induction of MN-PCE and the induction of SCE to the lack of a significant correlation between the depression in the MI and any other endpoint.

Research Organization:
Brookhaven National Lab., Upton, NY
DOE Contract Number:
AC02-76CH00016
OSTI ID:
5844458
Journal Information:
Environ. Mutagen.; (United States), Journal Name: Environ. Mutagen.; (United States) Vol. 9:3; ISSN ENMUD
Country of Publication:
United States
Language:
English

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Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL TISSUES
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGY
BODY
BONE MARROW
BUTADIENE
CELL CONSTITUENTS
CELL NUCLEI
CHROMOSOMAL ABERRATIONS
CHRONIC EXPOSURE
CYTOLOGY
DIENES
GENETIC EFFECTS
HEMATOPOIETIC SYSTEM
HYDROCARBONS
MALES
MAMMALS
MICE
MITOTIC INDEX
MUTATIONS
ORGANIC COMPOUNDS
ORGANS
POLYENES
RODENTS
SISTER CHROMATID EXCHANGES
TISSUES
VERTEBRATES