Effect of acrylamide on hepatocellular DNA repair

Journal Article · Wed Jan 01 04:00:00 EST 1986 · Environ. Mutagen.; (United States)

DOI:https://doi.org/10.1002/em.2860080109· OSTI ID:5842932

Miller, M J; McQueen, C A

Acrylamide has recently been reported to induce tumors in laboratory animals. The effect of acrylamide on unscheduled DNA synthesis using the hepatocyte primary culture (HPC)/DNA repair test was examined. Isolated hepatocytes were exposed to acrylamide and (3H)thymidine ( (3H)TdR) for 18 hr. Incorporation of (3H)TdR into DNA was determined by autoradiography. No DNA repair was observed at acrylamide concentrations up to 10(-2) M. These findings were confirmed using density gradients. Acrylamide concentrations exceeding 10(-2) M were cytotoxic to hepatocytes. Because both autoradiography and density gradients measure DNA repair as an endpoint, the ability of acrylamide to inhibit these repair processes was also determined. Acrylamide had no effect on the repair of UV-damaged DNA. These results show that acrylamide is not genotoxic in isolated hepatocytes.

• Research Organization: American Health Foundation, Valhalla, NY
• OSTI ID: 5842932
• Journal Information: Environ. Mutagen.; (United States), Journal Name: Environ. Mutagen.; (United States) Vol. 1; ISSN ENMUD
• Country of Publication: United States
• Language: English

Related Subjects

560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY
DIGESTIVE SYSTEM
DISEASES
DNA REPAIR
GLANDS
HETEROCYCLIC COMPOUNDS
LABELLED COMPOUNDS
LIVER
LIVER CELLS
MAMMALS
MUTAGENS
NEOPLASMS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC POLYMERS
ORGANS
POLYAMIDES
POLYMERS
PYRIMIDINES
RATS
RECOVERY
REPAIR
RIBOSIDES
RODENTS
SOMATIC CELLS
THYMIDINE
TRITIUM COMPOUNDS
UPTAKE
VERTEBRATES