Assay to detect chemically induced DNA repair in rat spermatocytes
Journal Article
·
· Environ. Mutagen.; (United States)
An in vivo/in vitro DNA repair assay has been developed to quantitate chemically induced unscheduled DNA synthesis (UDS) in rat spermatocytes utilizing autoradiography. Male Fischer-344 rats were treated by i.p. injection or gavage with a variety of genotoxic agents dissolved in dimethyl sulfoxide, corn oil, or water. At selected times after treatment, spermatocytes were isolated by trypsin digestion of testes and cultured for 24 hr in the presence of /sup 3/H-thymidine. The direct-acting genotoxicants methyl methanesulfonate (MMS) and ethyl methanesulfonate and the chemotherapeutic agent cyclophosphamide (CPA) produced positive UDS responses in spermatocyes isolated l hr after i.p. injection. Other known genotoxicants--including dimethylnitrosamine, aflatoxin B/sub 1/, 2-acetylaminofluorene, 2, 6-dinitrotoluene, and l,6-dinitropyrene--failed to induce UDS, even with routes of administration and at times of exposure known to produce a positive response in hepatocytes. These results demonstrate that the in vivo/in vitro spermatocyte DNA repair assay may be useful as a predictive screen for germ cell mutagens.
- Research Organization:
- Chemical Industry Inst. of Toxicology, Research Triangle Park, NC
- OSTI ID:
- 6625320
- Journal Information:
- Environ. Mutagen.; (United States), Journal Name: Environ. Mutagen.; (United States) Vol. 6:3; ISSN ENMUD
- Country of Publication:
- United States
- Language:
- English
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Journal Article
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Sat Dec 31 23:00:00 EST 1983
· Environ. Mutagen.; (United States)
·
OSTI ID:6593949
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Sat Dec 31 23:00:00 EST 1988
· Environmental and Molecular Mutagenesis; (USA)
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OSTI ID:6866738
Measurement of unscheduled DNA synthesis in germ cells of the male mouse (Mus musculus)
Conference
·
Mon Dec 31 23:00:00 EST 1984
·
OSTI ID:5516999
Related Subjects
560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKYLATING AGENTS
ANIMALS
AUTORADIOGRAPHY
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DATA
DNA
DRUGS
EMS
ENDOXAN
ESTERS
EXPERIMENTAL DATA
GERM CELLS
HETEROCYCLIC COMPOUNDS
IMMUNOSUPPRESSIVE DRUGS
INFORMATION
LABELLED COMPOUNDS
MAMMALS
METHYL METHANESULFONATE
MUTAGENS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PYRIMIDINES
RATS
RECOVERY
REPAIR
RIBOSIDES
RODENTS
SPERMATOCYTES
SULFONIC ACID ESTERS
THYMIDINE
TRITIUM COMPOUNDS
VERTEBRATES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKYLATING AGENTS
ANIMALS
AUTORADIOGRAPHY
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DATA
DNA
DRUGS
EMS
ENDOXAN
ESTERS
EXPERIMENTAL DATA
GERM CELLS
HETEROCYCLIC COMPOUNDS
IMMUNOSUPPRESSIVE DRUGS
INFORMATION
LABELLED COMPOUNDS
MAMMALS
METHYL METHANESULFONATE
MUTAGENS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PYRIMIDINES
RATS
RECOVERY
REPAIR
RIBOSIDES
RODENTS
SPERMATOCYTES
SULFONIC ACID ESTERS
THYMIDINE
TRITIUM COMPOUNDS
VERTEBRATES