Biochemical and biophysical studies of the E. coli aerobic respiratory chain: Progress report, (September 1, 1981-August 31, 1982 and September 1, 1982-August 31, 1983)

Technical Report · Sat Jan 01 04:00:00 EST 1983

OSTI ID:5842375

Gennis, R B

These studies demonstrate that organization of the E. coli aerobic respiratory chain is relatively simple. Over half of the spectroscopically defined cytochromes in E. coli are part of a single complex containing only two kinds of polypeptides. It should be possible to dissect all the parts of the respiratory chain required for efficient electron transport as well as for bioenergetic transduction. Further it is possible to use reconstitution techniques to assay in vitro for the functions of the various purified components. A mutant lacking the low aeration branch of the cytochrome chain has been isolated. It should be possible to obtain a complete genetic analysis of every component required for respiratory growth in E. coli. 8 figs.

• Research Organization: Illinois Univ., Urbana (USA). Dept. of Chemistry
• DOE Contract Number: AC02-80ER10682
• OSTI ID: 5842375
• Report Number(s): DOE/ER/10682-T3; ON: DE88000905
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ABSORPTION SPECTROSCOPY
BACTERIA
BIOCHEMICAL REACTION KINETICS
CHROMATOGRAPHY
CYTOCHROMES
DOCUMENT TYPES
ELECTROPHORESIS
ESCHERICHIA COLI
FRACTIONATION
GEL PERMEATION CHROMATOGRAPHY
GENE MUTATIONS
IMMUNOASSAY
KINETICS
MEMBRANE PROTEINS
MICROORGANISMS
MUTATIONS
NITROSO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PIGMENTS
PROGRESS REPORT
PROTEINS
REACTION KINETICS
SEPARATION PROCESSES
SPECTROSCOPY