Biochemical and biophysical studies of the E. coli aerobic respiratory chain: Annual performance report for the period September 1, 1986 to August 31, 1987

Technical Report · Sat Aug 01 04:00:00 EDT 1987

OSTI ID:5857087

Gennis, R B

The molecular characterization of components of the aerobic respiratory chain of Escherichia coli was sought by study of the two terminal oxidase complexes, called the cytochrome o complex. We previously purified this enzyme and cloned the gene. Recently we have obtained the complete DNA sequence of this gene. Studies have been started to clone the gene encoding a second NADH dehydrogenase in E. coli. This is of interest to us because the enzyme must function as a coupling site or proton pump in the membrane.

• Research Organization: Illinois Univ., Urbana (USA)
• DOE Contract Number: AC02-80ER10682
• OSTI ID: 5857087
• Report Number(s): DOE/ER/10682-1; ON: DE88000153
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CLONING
CYTOCHROME OXIDASE
DAYS LIVING RADIOISOTOPES
DNA SEQUENCING
DNA-CLONING
DOCUMENT TYPES
ENZYMES
ESCHERICHIA COLI
EVEN-ODD NUCLEI
FRACTIONATION
GENE MUTATIONS
HAEM DEHYDROGENASES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MEMBRANE PROTEINS
MICROORGANISMS
MUTATIONS
NUCLEI
NUCLEOTIDE DEHYDROGENASES
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PROGRESS REPORT
PROTEINS
RADIOISOTOPES
SEPARATION PROCESSES
SPECIES DIVERSITY
STRUCTURAL CHEMICAL ANALYSIS
SULFUR 35
SULFUR ISOTOPES
TRACER TECHNIQUES