Biochemical and biophysical studies of the E. coli respiratory chain

Technical Report · Tue Jan 01 04:00:00 EST 1991

OSTI ID:5464494

Gennis, R B

E. coli contains two distinct ubiquinol oxidases but contains no cytochrome c-dependent branch of its aerobic respiratory chain. The cytochrome d complex has a very high affinity for molecular oxygen and is the predominant oxidase when the cells are grown with limited oxygen. The cytochrome o complex is the predominant oxidase present when E. coli is grown at high aeration. This grant is directed towards characterizing the cytochrome o complex. Substantial progress has been made during the past five years. The single most important results of our work is the demonstration that the E. coli cytochrome o complex is closely related structurally to the family of mitochondrial and bacterial aa{sub 3}-type cytochrome c oxidases. This has revealed the existence of a superfamily of proton-pumping respiratory oxidases that share common structural and mechanistic features, but vary with respect to substrate (cytochrome c vs quinol) and/or heme components (a-type vs protoheme). 55 refs., 8 figs.

• Research Organization: Illinois Univ., Urbana, IL (United States). Dept. of Chemistry
• Sponsoring Organization: DOE; USDOE, Washington, DC (United States)
• DOE Contract Number: FG02-87ER13716
• OSTI ID: 5464494
• Report Number(s): DOE/ER/13716-2; ON: DE91016966
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
550300 -- Cytology
550400 -- Genetics
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
BACTERIA
BARYONS
BIOCHEMICAL REACTION KINETICS
BIOCHEMISTRY
BIOLOGICAL PATHWAYS
BIOPHYSICS
BIOTECHNOLOGY
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CHEMISTRY
COPPER
CYTOCHROMES
DNA SEQUENCING
DOCUMENT TYPES
ELECTRON SPIN RESONANCE
ELEMENTARY PARTICLES
ELEMENTS
ENZYME ACTIVITY
ENZYME INDUCTION
ENZYMES
ESCHERICHIA COLI
FERMIONS
FOURIER TRANSFORM SPECTROMETERS
GENE REGULATION
HADRONS
HEME
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
KINETICS
MAGNETIC RESONANCE
MEASURING INSTRUMENTS
MEMBRANE PROTEINS
MEMBRANE TRANSPORT
METALS
MICROORGANISMS
MOLECULAR STRUCTURE
MUTAGENESIS
NUCLEONS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDOREDUCTASES
PIGMENTS
PORPHYRINS
PROGRESS REPORT
PROTEIN STRUCTURE
PROTEINS
PROTONS
PURIFICATION
REACTION KINETICS
REDOX REACTIONS
RESONANCE
SPECTROMETERS
STRUCTURAL CHEMICAL ANALYSIS
TRANSITION ELEMENTS