Biochemical and biophysical studies on the E. coli aerobic respiratory chain: Progress report, September 1, 1980-August 31, 1981

Technical Report · Fri Apr 17 04:00:00 EST 1981

OSTI ID:5857259

Gennis, R B

Studies to elucidate the aerobic electron transport chain in E. coli are reported. We have used analytical isoelectric focussing (IEF) to identify heme protein complexes in the cytoplasmic membrane of E. coli. The proteins have been separated according to their isoelectric points. Using membranes isolated from cells grown with very high aeration approximately 20 protein bands are clearly resolved, only one of which stains for heme.

• Research Organization: Illinois Univ., Urbana (USA)
• DOE Contract Number: AC02-80ER10682
• OSTI ID: 5857259
• Report Number(s): DOE/ER/10682-T2; ON: DE88000906
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANTIGEN-ANTIBODY REACTIONS
BACTERIA
CELL CONSTITUENTS
CYTOCHROMES
DOCUMENT TYPES
ELECTROPHORESIS
ESCHERICHIA COLI
FRACTIONATION
GENE MUTATIONS
MEMBRANE PROTEINS
MICROORGANISMS
MITOCHONDRIA
MUTATIONS
ORGANIC COMPOUNDS
ORGANOIDS
PIGMENTS
PROGRESS REPORT
PROTEINS
SEPARATION PROCESSES