Cloning and gene arrangement of PSAC, NDHE and NDHD from the cyanobacterium Synechocystis sp. PCC 6803
- Michigan State Univ., East Lansing (USA)
In higher plants the psaC gene, which encodes the 9 kDa 2(Fe-4S) containing Photosystem I subunit, is located in the small single copy region of the chloroplast DNA between two ORF's with homology to genes encoding subunits of the mitochondrial NADH Dehydrogenase complex. The psaC gene has been cloned from Synechocystis and analysis of the flanking sequences has shown the 5'ndhE-psaCndhD3{prime} arrangement of higher plants is only partially conserved in Synechocystis. An open reading frame 5{prime} to psaC has 79% identity to the maize ndhE gene. Downstream of psaC there is an open reading frame of only 273 bp with 48% identity to the 5{prime} end of the maize ndhD gene (1,545 bp). Southern analysis shows that psaC, ndhE and the region of homology to ndhD are present in only a single copy in the Synechocystis genome. Probes to the 3{prime} portion of the wheat ndhD gene did not hybridize to Synechocystis or Anabaena genomic DNA, but did hybridize to Oenothera chloroplast DNA, indicating the entire ndhD gene is not present in these cyanobacteria.
- OSTI ID:
- 5841398
- Report Number(s):
- CONF-9007196--
- Journal Information:
- Plant Physiology, Supplement; (USA), Journal Name: Plant Physiology, Supplement; (USA) Vol. 93:1; ISSN PPYSA; ISSN 0079-2241
- Country of Publication:
- United States
- Language:
- English
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