Human U1 snRNP-specific C protein: Complete cDNA and protein sequence and identification of a multigene family in mammals
- Univ. of Nijmegen (Netherlands)
A complementary DNA clone for the human U1 snRNP-specific C protein has been isolated. The nucleotide sequence of the 733 bp cDNA insert includes a 15 bp 5{prime}-untranslated region, an open reading frame of 477 bp corresponding to 159 amino acids (M{sub r}=17,373 D), and a 223 bp 3{prime}-untranslated region. The identity of the clone was confirmed by in vitro translation of hybrid-selected mRNA or an RNA transcript synthesized from the cDNA. The in vitro synthesized C protein has a slightly greater mobility on SDS-polyacrylamide gels, indicating that the in vivo product is post-translationally modified. Analysis of genomic DNA restriction enzyme digests shows hybridizing fragments in the genome of all vertebrate classes. The results are consistent with multi-copy representation of the C protein gene in mammals, whereas in the other vertebrate classes the related protein seems to be encoded by a single-copy gene.
- OSTI ID:
- 6949195
- Journal Information:
- Nucleic Acids Research; (UK), Journal Name: Nucleic Acids Research; (UK) Vol. 16:17; ISSN 0305-1048; ISSN NARHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
AMINES
AMINO ACID SEQUENCE
AMINO ACIDS
ANIMALS
ANTIBODIES
AZOLES
CARBOXYLIC ACIDS
DNA
DNA SEQUENCING
DRUGS
GENES
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
LIPOTROPIC FACTORS
MAMMALS
MAN
MESSENGER-RNA
METHIONINE
MOLECULAR STRUCTURE
MONOCLONAL ANTIBODIES
NUCLEIC ACIDS
NUCLEOPROTEINS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PRIMATES
PROLINE
PROTEINS
PYRROLES
PYRROLIDINES
RECOMBINANT DNA
RNA
STRUCTURAL CHEMICAL ANALYSIS
TRANSCRIPTION
VERTEBRATES