Membrane lipid peroxidation by UV-A: Mechanism and implications

Journal Article · Mon Oct 01 00:00:00 EDT 1990 · Biotechnology and Applied Biochemistry; (USA)

OSTI ID:5839482

Bose, B; Agarwal, S; Chatterjee, S N ^[1]

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1. Saha Institute of Nuclear Physics, Calcutta (India)

UV-A produced a dose-dependent linear increase of lipid peroxidation in liposomal membrane, as detected by the assay of (i) conjugated dienes, (ii) lipid hydroperoxides, (iii) malondialdehydes (MDA), and (iv) the fluorescent adducts formed by the reaction of MDA with glycine and also a linear dose-dependent increase of ({sup 14}C)glucose efflux from the liposomes. UV-A-induced MDA production could not be inhibited by any significant degree by sodium formate, dimethyl sulfoxide, EDTA, or superoxide dismutase but was very significantly inhibited by butylated hydroxytoluene, alpha-tocopherol, sodium azide, L-histidine, dimethylfuran, and beta-carotene. MDA formation increased with an increase in the D{sub 2}O content in water, leading to a maximal amount of nearly 50% enhancement of lipid peroxidation in 100% D{sub 2}O vis-a-vis water used as dispersion medium. The experimental findings indicate the involvement of singlet oxygen as the initiator of the UV-A-induced lipid peroxidation.

• OSTI ID: 5839482
• Journal Information: Biotechnology and Applied Biochemistry; (USA), Vol. 12:5; ISSN 0885-4513
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
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CHEMICAL RADIATION EFFECTS
ALDEHYDES
ANTIOXIDANTS
CARBON 14 COMPOUNDS
DEUTERIUM
DMSO
DOSE-RESPONSE RELATIONSHIPS
EDTA
FLUORESCENCE
GLUCOSE
LIPOSOMES
MEMBRANE PROTEINS
METABOLISM
RADICALS
SUPEROXIDE DISMUTASE
TRACER TECHNIQUES
ULTRAVIOLET RADIATION
AMINO ACIDS
CARBOHYDRATES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHELATING AGENTS
CHEMISTRY
ELECTROMAGNETIC RADIATION
ENZYMES
HEXOSES
HYDROGEN ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LUMINESCENCE
MONOSACCHARIDES
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANOIDS
OXIDOREDUCTASES
PROTEINS
RADIATION CHEMISTRY
RADIATION EFFECTS
RADIATIONS
SACCHARIDES
STABLE ISOTOPES
SULFOXIDES
560120* - Radiation Effects on Biochemicals
Cells
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550501 - Metabolism- Tracer Techniques