Membrane lipid peroxidation by UV-A: Mechanism and implications

Journal Article · Mon Oct 01 00:00:00 EDT 1990 · Biotechnology and Applied Biochemistry; (USA)

OSTI ID:5839482

Bose, B; Agarwal, S; Chatterjee, S N ^[1]

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1. Saha Institute of Nuclear Physics, Calcutta (India)

UV-A produced a dose-dependent linear increase of lipid peroxidation in liposomal membrane, as detected by the assay of (i) conjugated dienes, (ii) lipid hydroperoxides, (iii) malondialdehydes (MDA), and (iv) the fluorescent adducts formed by the reaction of MDA with glycine and also a linear dose-dependent increase of ({sup 14}C)glucose efflux from the liposomes. UV-A-induced MDA production could not be inhibited by any significant degree by sodium formate, dimethyl sulfoxide, EDTA, or superoxide dismutase but was very significantly inhibited by butylated hydroxytoluene, alpha-tocopherol, sodium azide, L-histidine, dimethylfuran, and beta-carotene. MDA formation increased with an increase in the D{sub 2}O content in water, leading to a maximal amount of nearly 50% enhancement of lipid peroxidation in 100% D{sub 2}O vis-a-vis water used as dispersion medium. The experimental findings indicate the involvement of singlet oxygen as the initiator of the UV-A-induced lipid peroxidation.

• OSTI ID: 5839482
• Journal Information: Biotechnology and Applied Biochemistry; (USA), Journal Name: Biotechnology and Applied Biochemistry; (USA) Vol. 12:5; ISSN BABIE; ISSN 0885-4513
• Country of Publication: United States
• Language: English

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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
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AMINO ACIDS
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