Effect of dilution on carboxymethylcellulase and xylanase assays

Journal Article · Wed Aug 01 04:00:00 EDT 1984 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260260825· OSTI ID:5833170

Cauchon, N; LeDuy, A

It has been demonstrated that the dilution of samples prior to the carboxymethylcellulase and xylanase assays causes serious discrepancies in the numerical values obtained for the enzyme activities. Even when the sample is assayed with the identical procedure, one could obtain different numerical values of the enzyme activity U depending on how much this sample has been diluted before the enzyme assay. Two crude commercial cellulase samples of Aspergillus niger and Trichoderma viride as well as the culture filtrate of our newly isolated acidophilic fungus have been used for the demonstration. An empirical method for reporting the cellulolytic activity by taking into account this dilution effect is proposed.

• Research Organization: Laval Univ., Sainte-Foy, Quebec
• OSTI ID: 5833170
• Journal Information: Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 26:8; ISSN BIBIA
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
140504 -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)
550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ASPERGILLUS
CELLULASE
CELLULOLYTIC ACTIVITY
CHEMICAL ANALYSIS
DILUTION
ENZYME ACTIVITY
ENZYMES
ERRORS
FUNGI
GLYCOSYL HYDROLASES
HYDROLASES
MEASURING METHODS
O-GLYCOSYL HYDROLASES
PLANTS
QUALITATIVE CHEMICAL ANALYSIS
TRICHODERMA
TRICHODERMA VIRIDE
XYLANASE