Pitfalls in the assay of carboxymethylcellulase activity. [Sclerotium rolfsii]
Journal Article
·
· Biotechnol. Bioeng.; (United States)
A purified endocellulase from Sclerotium rolfsii and a crude cellulase preparation from Trichoderma reesei are used to illustrate several pitfalls associated with the assay of carboxymethylcellulase activity and the subsequent attainment of linear enzyme dilution curves. It is shown that the nature of both the enzymes and the substrate make the assay unsuitable for use in the calculation of enzyme recovery and purity. (Refs. 16).
- Research Organization:
- Dept of Biochemistry, Box 375, Pietermaritzburg 3200, South Africa
- OSTI ID:
- 5794738
- Journal Information:
- Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 25:2; ISSN BIBIA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
09 BIOMASS FUELS
140504* -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
CELLULASE
CELLULOLYTIC ACTIVITY
ENZYME ACTIVITY
ENZYMES
ERRORS
FUNGI
GLYCOSYL HYDROLASES
HYDROLASES
MANAGEMENT
MATERIALS RECOVERY
MEASURING METHODS
O-GLYCOSYL HYDROLASES
PLANTS
PROCESSING
PURIFICATION
RECOVERY
TRICHODERMA
TRICHODERMA VIRIDE
WASTE MANAGEMENT
WASTE PROCESSING
140504* -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
CELLULASE
CELLULOLYTIC ACTIVITY
ENZYME ACTIVITY
ENZYMES
ERRORS
FUNGI
GLYCOSYL HYDROLASES
HYDROLASES
MANAGEMENT
MATERIALS RECOVERY
MEASURING METHODS
O-GLYCOSYL HYDROLASES
PLANTS
PROCESSING
PURIFICATION
RECOVERY
TRICHODERMA
TRICHODERMA VIRIDE
WASTE MANAGEMENT
WASTE PROCESSING