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Selective systems for obtaining recessive ribosomal suppressors in Saccharomyces yeast

Journal Article · · Sov. Genet. (Engl. Transl.); (United States)
OSTI ID:5826586
; ;

The absolute selectivity of the system used for the selection of recessive mutations in only two genes (ribosomal suppressors sup1 and sup2) is based on the obtaining of simultaneous reversions to prototrophy for adenine and histidine in Saccharomyces cerevisiae haploids carrying two different nonsense mutations: his7-1 (UAA) and adel-14 (UGA, identified in this study). The selectivity of the system is determined not only by the use of different types of nonsense but also by the selection of actual nonsense alleles, and also by the genotypic background of the culture. The lys2 mutants were induced by UV irradiation (dose 300 J/m/sup 2/) of the strains on the AAA medium, selective for the growth of these mutants.

Research Organization:
A.A. Zhdanov State Univ., Leningrad (USSR)
OSTI ID:
5826586
Journal Information:
Sov. Genet. (Engl. Transl.); (United States), Journal Name: Sov. Genet. (Engl. Transl.); (United States) Vol. 24:7; ISSN SOGEB
Country of Publication:
United States
Language:
English

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Related Subjects

550400* -- Genetics
550700 -- Microbiology
560400 -- Other Environmental Pollutant Effects
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADENINES
AMINES
AMINO ACIDS
ANTIMETABOLITES
AROMATICS
AZAARENES
AZOLES
BIOLOGICAL EFFECTS
BIOSYNTHESIS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
DRUGS
ELECTROMAGNETIC RADIATION
EUMYCOTA
FUNGI
GENE MUTATIONS
GENETIC EFFECTS
GENOTYPE
HAPLOIDY
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HISTIDINE
HYBRIDIZATION
IMIDAZOLES
INHIBITION
LYSINE
MICROORGANISMS
MUTAGENESIS
MUTANTS
MUTATIONS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANOIDS
PHENOTYPE
PLANTS
PLOIDY
PROGENY
PURINES
RADIATIONS
RECESSIVE MUTATIONS
REVERTANTS
RIBOSOMES
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
SYNTHESIS
ULTRAVIOLET RADIATION
YEASTS