Selective systems for obtaining recessive ribosomal suppressors in Saccharomyces yeast
The absolute selectivity of the system used for the selection of recessive mutations in only two genes (ribosomal suppressors sup1 and sup2) is based on the obtaining of simultaneous reversions to prototrophy for adenine and histidine in Saccharomyces cerevisiae haploids carrying two different nonsense mutations: his7-1 (UAA) and adel-14 (UGA, identified in this study). The selectivity of the system is determined not only by the use of different types of nonsense but also by the selection of actual nonsense alleles, and also by the genotypic background of the culture. The lys2 mutants were induced by UV irradiation (dose 300 J/m/sup 2/) of the strains on the AAA medium, selective for the growth of these mutants.
- Research Organization:
- A.A. Zhdanov State Univ., Leningrad (USSR)
- OSTI ID:
- 5826586
- Journal Information:
- Sov. Genet. (Engl. Transl.); (United States), Vol. 24:7; Other Information: Translated from Genetika; 24: No. 7, 1159-1165(Jul 1988)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
GENE MUTATIONS
GENOTYPE
RECESSIVE MUTATIONS
REVERTANTS
SACCHAROMYCES CEREVISIAE
MUTAGENESIS
ULTRAVIOLET RADIATION
GENETIC EFFECTS
ADENINES
BIOSYNTHESIS
HAPLOIDY
HISTIDINE
HYBRIDIZATION
INHIBITION
LYSINE
MUTANTS
PHENOTYPE
PROGENY
RIBOSOMES
AMINES
AMINO ACIDS
ANTIMETABOLITES
AROMATICS
AZAARENES
AZOLES
BIOLOGICAL EFFECTS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
DRUGS
ELECTROMAGNETIC RADIATION
EUMYCOTA
FUNGI
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
IMIDAZOLES
MICROORGANISMS
MUTATIONS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANOIDS
PLANTS
PLOIDY
PURINES
RADIATIONS
SACCHAROMYCES
SYNTHESIS
YEASTS
550400* - Genetics
550700 - Microbiology
560400 - Other Environmental Pollutant Effects