Nonsense suppressors of yeast cause osmotic-sensitive growth. [Saccharomyces cerevisiae]

Journal Article · Sat Jan 01 00:00:00 EST 1977 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.74.1.305· OSTI ID:7224298

Singh, A

Many nonsense suppressors of Saccharomyces cerevisiae cause growth inhibition on hypertonic media. Eight tyrosine-inserting UAA (ochre) suppressors, eight tyrosine-inserting UAG (amber) suppressors, a leucine-inserting UAG suppressor, and a serine-inserting recessive lethal UAG suppressor cause osmotic sensitivity whereas a serine-inserting UAA suppressor does not cause sensitivity. Although the mechanism is not understood, the growth inhibition of specific suppressors on hypertonic media is correlated with their efficiencies of suppression. This heretofore unknown property of nonsense suppressors is useful for mitotic mapping, selecting tRNA mutants, selecting antisuppressors, and scoring nonsense suppressors.

• Research Organization: Univ. of Rochester, NY (United States)
• OSTI ID: 7224298
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 74:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
SACCHAROMYCES CEREVISIAE
GROWTH
MUTATIONS
GENETICS
YEASTS
BIOLOGY
BIOMASS
ENERGY SOURCES
FUNGI
MICROORGANISMS
PLANTS
RENEWABLE ENERGY SOURCES
SACCHAROMYCES
550400* - Genetics