Patterns of genetic and phenotypic suppression of lys2 mutations in the yeast Saccharomyces cerevisiae
A total of 358 lys2 mutants of Saccharomyces cerevisiae have been characterized for suppressibility by the following suppressors: UAA and UAG suppressors that insert tyrosine, serine, or leucine; a putative UGA suppressor; an omnipotent suppressor SUP46; and a frameshift suppressor SUF1-1. In addition, the lys2 mutants were examined for phenotypic suppression by the aminoglycoside antibiotic paromomycin, for osmotic remediability and for temperature sensitivity. The mutants exhibited over 50 different patterns of suppression and most of the nonsense mutants appeared similar to nonsense mutants previously described. A total of 24% were suppressible by one or more of the UAA suppressors, 4% were suppressible by one or more of the UAG suppressors, while only one was suppressible by the UGA suppressor and only one was weakly suppressible by the frameshift suppressor. One mutant responded to both UAA and UAG suppressors, indicating that UAA or UAG mutations at certain rare sites can be exceptions to the specific action of UAA and UAG suppressors. Some of the mutants appeared to require certain types of amino acid replacements at the mutant sites in order to produce a functional gene product, while others appeared to require suppressors that were expressed at high levels. Many of the mutants suppressible by SUP46 and paromomycin were not suppressible by any of the UAA, UAG, or UGA suppressors, indicating that omnipotent suppression and phenotypic suppression need not be restricted to nonsense mutations. All of the mutants suppressible by SUP46 were also suppressible by paromomycin, suggesting a common mode of action of omnipotent suppression and phenotypic misreading.
- Research Organization:
- Univ. of Rochester, NY
- OSTI ID:
- 5504871
- Journal Information:
- Genetics; (United States), Journal Name: Genetics; (United States) Vol. 93:1; ISSN GENTA
- Country of Publication:
- United States
- Language:
- English
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550400* -- Genetics
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59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANTIBIOTICS
BIOLOGICAL EFFECTS
BIOLOGICAL VARIABILITY
CARBOXYLIC ACIDS
CODONS
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ENZYME INHIBITORS
FUNGI
GENES
GENETIC VARIABILITY
GROWTH
LYSINE
MICROORGANISMS
MUTATIONS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHENOTYPE
PLANTS
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
YEASTS