Effect of heat shock on the intracellular distribution of proteins

Journal Article · Fri Mar 01 04:00:00 EST 1985 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576507· OSTI ID:5825219

Murnane, J P; Li, G C

Exposure of cells to heat induces thermotolerance, a transient resistance to subsequent heat challenges. It has been shown that thermotolerance is correlated in time with the enhanced synthesis of heat shock proteins. In this study, the association of induced heat shock proteins with various cellular fractions was investigated and the heat-induced changes in skeletal protein composition in thermotolerant and control cells was compared. All three major heat shock proteins induced in Chinese hamster fibroblasts after a 46/sup 0/C, 4-min heat treatment (70, 87, and 110 kDa) were purified with the cytoplasmic fraction, whereas only the 70-kDa protein was also found in other cell fractions, including that containing the cellular skeleton. Immediately after a second heat treatment at 45/sup 0/C for 45 min, the 110-kDa protein from thermotolerant cells also purified extensively with the cellular skeletal fraction. The alteration or recovery kinetics of the total labeled protein content of the cellular skeletal fraction after heat shock did not correlate with the dramatic increase in survival observed in thermotolerant cells. The relationship between heat shock proteins and thermotolerance, therefore, does not correlate directly with changes in the heat-induced cellular alterations leading to differences in protein fractionation.

• Research Organization: Univ. of California, San Francisco
• DOE Contract Number: AC03-76SF01012
• OSTI ID: 5825219
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 101:3; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

550301 -- Cytology-- Tracer Techniques
560201* -- Thermal Effects-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL ADAPTATION
BIOSYNTHESIS
CARBOXYLIC ACIDS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DISTRIBUTION
DRUGS
ELECTROPHORESIS
EVEN-ODD NUCLEI
FIBROBLASTS
HAMSTERS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MAMMALS
METHIONINE
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PROTEINS
RADIOISOTOPES
RODENTS
SOMATIC CELLS
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
TEMPERATURE EFFECTS
TRACER TECHNIQUES
VERTEBRATES