Acquired thermotolerance and heat shock proteins in thermophiles form the three phylogenetic domains

Journal Article · Sat Oct 01 04:00:00 EDT 1994 · Journal of Bacteriology

OSTI ID:136722

Trent, J D ^[1]; Gabrielsen, M; Jensen, B; Neuhard, J; Olsen, J ^[2]

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1. Argonne National Lab., IL (United States). Center for Mechanistic Biology and Biotechnology
2. Univ. of Copenhagen (Denmark). Institute of Molecular Biology

Thermophilic organisms from each of the three phylogenetic domains (Bacteria, Archaea, and Eucarya) acquired thermotolerance after heat shock. Bacillus caldolyticus grown at 60 C and heat shocked at 69 C for 10 min showed thermotolerance at 74 C, Sulfolobus shibatae grown at 70 C and heat shocked at 88 C for 60 min showed thermotolerance at 95 C, and Thermomyces lanuginosus grown at 50 C and hat shocked at 55 C for 60 min showed thermotolerance at 58 C. Determinations of protein synthesis during heat shock revealed differences in the dominant heat shock proteins for each species. For B. caldolyticus, a 70-kDa protein dominated while for S. shibatae, a 55-kDa protein dominated and for T. lanuginosus, 31- to 33-kDa proteins dominated. Reagents that disrupted normal protein synthesis during heat shock prevented the enhanced thermotolerance.

• Research Organization: Argonne National Laboratory (ANL), Argonne, IL
• DOE Contract Number: W-31109-ENG-38
• OSTI ID: 136722
• Journal Information: Journal of Bacteriology, Journal Name: Journal of Bacteriology Journal Issue: 19 Vol. 176; ISSN JOBAAY; ISSN 0021-9193
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
BACTERIA
BIOLOGICAL ADAPTATION
BIOSYNTHESIS
EXPERIMENTAL DATA
GENETIC VARIABILITY
HEAT-SHOCK PROTEINS
TOLERANCE