Pharmacologically different Na/H antiporters on the apical and basolateral surfaces of cultured porcine kidney cells (LLC-PK/sub 1/)

Journal Article · Thu Sep 01 04:00:00 EDT 1988 · Proc. Natl. Acad. Sci. USA; (United States)

DOI:https://doi.org/10.1073/pnas.85.18.6797· OSTI ID:5822788

Haggerty, J G; Agarwal, N; Reilly, R F; Adelberg, E A; Slayman, C W

Proximal tubule cells of the kidney contain, on their apical surface, an amiloride-sensitive Na/H antiporter that functions in Na reabsorption and proton secretion. The authors have investigated the localization of the antiporter in a cloned cell line of porcine renal origin, LLC-PK/sub 1//Cl/sub 4/, which is often considered to be a useful model of the proximal tubule. Transport measurements were performed with differentiated monolayers grown on Nuclepore filters, permitting independent access to the apical and basolateral cell surfaces. The monolayers displayed significant Na/H antiporter activity (assayed as ethylisopropylamiloride-sensitive /sup 22/Na uptake) at both cell surfaces, with an apical uptake rate amounting to 44% and a basolateral rate amounting to 56% of the total. Significantly, the apical and basolateral antiporters could readily be distinguished from one another on the basis of ethylisopropylamiloride sensitivity. The PKE 20 mutant was found to overexpress the more resistant antiporter both during rapid growth and on its apical cell surface at confluence; normal amounts of the more sensitive antiporter were seen on the basolateral surface of confluent PKE 20 cells. Taken together, these results suggest that there are two distinct forms of the Na/H antiporter which are under separate genetic control.

• Research Organization: Yale Univ. School of Medicine, New Haven, CT (USA)
• OSTI ID: 5822788
• Journal Information: Proc. Natl. Acad. Sci. USA; (United States), Journal Name: Proc. Natl. Acad. Sci. USA; (United States) Vol. 85:18; ISSN PNASA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL COMPOUNDS
ALKALI METAL ISOTOPES
ANIMAL CELLS
ANIMALS
BARYONS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BODY
CARBON 14 COMPOUNDS
CATIONS
CELL CONSTITUENTS
CELL MEMBRANES
CHARGED PARTICLES
CHEMISTRY
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
ELEMENTARY PARTICLES
FERMIONS
HADRONS
INTERMEDIATE MASS NUCLEI
IONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
KIDNEYS
LABELLED COMPOUNDS
LIGHT NUCLEI
MAMMALS
MEMBRANE PROTEINS
MEMBRANE TRANSPORT
MEMBRANES
MINUTES LIVING RADIOISOTOPES
MUTANTS
NUCLEI
NUCLEONS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PERMEABILITY
PHARMACOLOGY
PROTEINS
PROTONS
RADIOISOTOPES
RECEPTORS
RUBIDIUM 86
RUBIDIUM ISOTOPES
SODIUM 22
SODIUM COMPOUNDS
SODIUM ISOTOPES
SOMATIC CELLS
SWINE
TRITIUM COMPOUNDS
TUBULES
UPTAKE
VERTEBRATES
YEARS LIVING RADIOISOTOPES