Inhibition of Na/sup +/-dependent uptake of hexose and amino acids in LLC-PK1 cells by amiloride and amiloride-derivatives

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5388270

Cook, J S; Shaffer, C; Cragoe, Jr, E J

Post-confluent LLC-PK1 cells, a renal epithelial cell line from pig kidney, have at their apical borders a Na/sup +/-dependent transport system for hexoses (assayed by the phlorizin-sensitive uptake of alpha-methylglucoside) and at their basolateral surfaces a Na/sup +/-dependent A-system transporter for amino acids (assayed by the uptake of methylaminoisobutyric acid). Amiloride at high concentrations (4.5 mM) partially inhibits both of these transporters. The analogs 5-(N-methyl-N-isobutyl) amiloride and 5-(N-ethyl-N-isopropyl) amiloride can completely inhibit both systems with inhibitory coefficients of approximately 50 ..mu..M in 150 mM NaCl media. Na/sup +//H/sup +/ exchange is also inhibited in these cells by these compounds, but at much lower concentrations. The inhibition of transport of the hexose is competitive with Na/sup +/ in the assay medium at Na/sup +/ concentrations below 10 mM.

• Research Organization: Oak Ridge National Lab., TN
• DOE Contract Number: AC05-84OR21400
• OSTI ID: 5388270
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

Related Subjects

560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALI METAL COMPOUNDS
AMINO ACIDS
ANIMAL TISSUES
ANIMALS
BIOLOGICAL EFFECTS
BODY
CARBOHYDRATES
CARBOXYLIC ACIDS
DIURETICS
DOMESTIC ANIMALS
DRUGS
EPITHELIUM
HEXOSES
INHIBITION
ION EXCHANGE
KIDNEYS
MAMMALS
MEMBRANE TRANSPORT
MONOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
SACCHARIDES
SODIUM COMPOUNDS
SWINE
TISSUES
UPTAKE
VERTEBRATES