Development of Na/sup +/-dependent hexose transport in a cultured line of porcine kidney cells

Journal Article · Fri Jan 01 04:00:00 EST 1982 · Am. J. Physiol.; (United States)

OSTI ID:5673863

Amsler, K; Cook, J S

LLC-PK/sub 1/ cells in culture do not concentrate ..cap alpha..-methylglucoside (..cap alpha..-meG) during their early growth phase but develop the capacity to concentrate this hexose as the growth rate decreases in confluent cultures. The concentration ability is dependent on the Na/sup +/ electrochemical gradient and is inhibited by phlorizin with K/sub 1,0,5/ approx. = 0.2 ..mu..M. The development of the concentrative capacity can be accelerated by the Friend cell inducer hexamethylene bisacetamide (HMBA) and by the phosphodiesterase inhibitors dibutyryl cAMP, theophyline, and 1-methyl-3-isobutylxanthine (MIX). In cultures treated with any of these differentiation-accelerating chemicals, the development of ..cap alpha..-meG concentrating capacity is severely inhibited by the tumor promoter 12-O-tetradecanoylphorbal-13-acetate (TPA) but not by inactive (in tumor promotion) analogs of TPA. In all cases, an early event in the development of ..cap alpha..-meG accumulating capacity is an elevated intracellular cAMP concentration; however the results suggest that this increase in cAMP may be necessary but not sufficient to induce the differentiated hexose-accumulating capacity.

• Research Organization: Univ. of Tennessee-Oak Ridge Graduate School of Biomedical Sciences
• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 5673863
• Journal Information: Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 242:1; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL COMPOUNDS
ANIMALS
BODY
CARBOHYDRATES
CATIONS
CELL CULTURES
CELL DIFFERENTIATION
CHARGED PARTICLES
DOMESTIC ANIMALS
HEXOSES
IONS
KIDNEYS
MAMMALS
MEMBRANE TRANSPORT
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANS
SACCHARIDES
SODIUM COMPOUNDS
SWINE
UPTAKE
VERTEBRATES