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Linear relationship of phlorizin-binding capacity and hexose uptake during differentiation in a clone of LLC-PK/sub 1/ cells

Journal Article · · J. Cell. Physiol.; (United States)
;
With a clone of (Cl 4) of LLC-PK cells, which develop a high capacity for Na/sup +/-dependent hexose uptake over time (days) in culture, we show that increasing uptake capacity is paralleled by an increase in the number of phlorizin-binding sites in the population. The linear relationship between binding and hexose transport is the same whether the cells differentiate spontaneously or are induced by either methylisobutylxanthine or hexamethylene bisacetamide. The constancy of the relationship suggests that the primary factor in transport development is the number of transporters in the cells rather than other possible factors like a change in membrane potential or decreased efflux. The K/sub d/ for phlorizin binding is .08 +/- .04 ..mu..M, and corresponds to K/sub i/ of 0.10 ..mu..M for transport inhibition. The turnover number of the transporter is estimated to be 170 +/- 40 molecules per second of ..cap alpha..-methyl glucoside.
Research Organization:
Oak Ridge National Lab., TN
DOE Contract Number:
AC05-84OR21400
OSTI ID:
5831002
Journal Information:
J. Cell. Physiol.; (United States), Journal Name: J. Cell. Physiol.; (United States) Vol. 122:2; ISSN JCLLA
Country of Publication:
United States
Language:
English

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Related Subjects

550200 -- Biochemistry
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACETAMIDE
AMIDES
ANIMAL TISSUES
AROMATICS
AZAARENES
BIOLOGICAL EFFECTS
BODY
CARBOHYDRATES
CELL CULTURES
CELL DIFFERENTIATION
CHEMICAL BONDS
CLONE CELLS
EPITHELIUM
GLYCOSIDES
HETEROCYCLIC COMPOUNDS
HEXOSES
KETONES
MEMBRANE TRANSPORT
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PHLORIZIN
PURINES
SACCHARIDES
TISSUES
UPTAKE
XANTHINES