Transport of tetraethylammonium by a kidney cell line (LLC-PK sub 1 )

Journal Article · Tue Mar 01 04:00:00 EST 1988 · American Journal of Physiology; (USA)

OSTI ID:6810215

Fauth, C; Rossier, B; Roch-Ramel, F ^[1]

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1. Institut de Pharmacologie de l'Universite de Lausanne (Switzerland)

The authors investigated whether the LLC-PK{sub 1} epithelial cell lines (which shows many characteristics of proximal tubular cells) also is capable of transporting an organic ion. Suspended LLC-PK{sub 1} cells accumulated tetraethylammonium (TEA). The uptake showed characteristics of a facilitated mechanism; TEA uptake was saturable and temperature-dependent and was inhibited by other organic cations. Quinine and mepiperphenidol were the most potent inhibitors, whereas N{sup 1}-methylnicotinamide and morphine inhibited the transport system only slightly at doses of 10{sup {minus}3} M. Basolateral-to-apical TEA flux through LLC-PK{sub 1} monolayers was five to six times larger than that of mannitol, a nontransported compound, whereas apical-to-basolateral TEA and mannitol fluxes were equal. Only the basolateral-to-apical TEA flux was inhibited by quinine. Under similar experimental conditions, no transport of p-aminohippuric acid was observed. It is concluded that LLC-PK{sub 1} cells are able to transport TEA, as do cells of the proximal tubule.

• OSTI ID: 6810215
• Journal Information: American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 254:3; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMMONIUM COMPOUNDS
ANIMAL CELLS
ANIMAL TISSUES
BIOCHEMICAL REACTION KINETICS
BODY
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CATIONS
CHARGED PARTICLES
DRUGS
EPITHELIUM
GLYCOSIDES
INHIBITION
IONS
KIDNEYS
KINETICS
LABELLED COMPOUNDS
MEMBRANE TRANSPORT
ORGANIC COMPOUNDS
ORGANS
OUABAIN
REACTION KINETICS
STROPHANTHINS
TEMPERATURE EFFECTS
TISSUES
UPTAKE