Identification of the receptor for erythropoietin on human and murine erythroleukemia cells and modulation by phorbol ester and dimethyl sulfoxide

Journal Article · Thu Sep 01 04:00:00 EDT 1988 · Proc. Natl. Acad. Sci. USA; (United States)

DOI:https://doi.org/10.1073/pnas.85.17.6513· OSTI ID:5821969

Broudy, V C; Lin, N; Egrie, J; De Haen, C; Weiss, T; Papayannopoulou, T; Adamson, J W

Erythropoietin, a glycoprotein that regulates erythropoiesis, initiates its biological effects by binding to a cell-surface receptor. Little is known about the structure of the erythropoietin receptor and the events that follow binding of erythropoietin to its receptor, in part because of the difficulty of obtaining sufficient quantities of cells that express the erythropoietin receptor. The authors used both iodinated and metabolically labeled erythropoietin to characterize the receptor on a variety of erythroleukemia cell lines not previously tested, and they have identified both human and murine cell lines that display large numbers of erythropoietin receptors. Both erythropoietin-responsive and -nonresponsive cell lines exhibit a single class of binding sites. Induction of the erythroid phenotype by dimethylsulfoxide or its suppression by phorbol 12-myristate 13-acetate was accompanied by an increase or decrease, respectively, in erythropoietin receptor number. Affinity crosslinking of /sup 125/I labeled erythropoietin to the receptor identified two proteins corresponding to estimated molecular masses of 95 and 105 kDa. The OCIM1, Rauscher, and GM979 erythroleukemia cell lines provide a useful model for the study of postreceptor signaling events, as well as a convenient source for purification of the erythropoietin receptor.

• Research Organization: Univ. of Washington, Seattle (USA)
• OSTI ID: 5821969
• Journal Information: Proc. Natl. Acad. Sci. USA; (United States), Journal Name: Proc. Natl. Acad. Sci. USA; (United States) Vol. 85:17; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
CARBOHYDRATES
CARBOXYLIC ACIDS
CARCINOGENS
CHEMICAL REACTIONS
CROSS-LINKING
DAYS LIVING RADIOISOTOPES
DMSO
ERYTHROPOIETIN
ESTERS
EVEN-ODD NUCLEI
GLUCOPROTEINS
GLYCOPROTEINS
INTERMEDIATE MASS NUCLEI
IRON 59
IRON ISOTOPES
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LYSINE
MEMBRANE PROTEINS
MITOGENS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHENOTYPE
PHORBOL ESTERS
POLYMERIZATION
PROTEINS
PURIFICATION
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
SACCHARIDES
SULFOXIDES
TRITIUM COMPOUNDS
TUMOR CELLS
UPTAKE