Specific binding of erythropoietin to its receptor on responsive mouse erythroleukemia cells

Journal Article · Mon Jun 01 04:00:00 EDT 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.12.4126· OSTI ID:5354010

Todokoro, K; Kanazawa, S; Amanuma, H; Ikawa, Y

Erythropoietin (Epo) is a glycoprotein factor that specifically regulates the proliferation and differentiation of erythroid progenitor cells. Here we describe the isolation of Epo-responsive mouse erythroleukemia cell line SKT6, the characterization of the specific binding of biologically active /sup 125/I-labeled human Epo (/sup 125/I-Epo) to it membrane receptor, and, finally, report information concerning the molecular structure of the receptor. About 75% of erythroid colony-forming precursor cell-like colonies derived from SKT6 cells were hemoglobin-positive after 3- to 4-day exposure to Epo in methylcellulose culture. Radioiodinated Epo bound specifically to SKT6 cells, and Scatchard analysis of the data showed high affinity for /sup 125/I-Epo but displayed only a small number of specific receptors. Membrane components that specifically interact with /sup 125/I-Epo were identified by covalent crosslinking with disuccinimidyl suberate, and three receptor species with apparent M/sub r/ 63,000, 94,000, and 119,000 were found in membrane from SKT6 cells, suggesting the complex structure of the receptor molecules. Specific bindings were also detected in all of the Epo-unresponsive Friend erythroleukemia cells examined, and cross-linking study revealed the presence of only the 63,000 species as a binding site.

• Research Organization: Institute of Physical and Chemical Research (RIKEN), Ibaraki, Japan
• OSTI ID: 5354010
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:12; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BLOOD FORMATION
CARBOHYDRATES
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
COLONY FORMATION
CROSS-LINKING
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ERYTHROPOIETIN
GLOBIN
GLUCOPROTEINS
HALOGENATION
HEMOGLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HORMONES
INTERMEDIATE MASS NUCLEI
IODINATION
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MICE
MOLECULAR WEIGHT
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEPTIDE HORMONES
PIGMENTS
POLYMERIZATION
PORPHYRINS
PROTEINS
RADIOISOTOPES
RADIORECEPTOR ASSAY
RODENTS
SACCHARIDES
TRACER TECHNIQUES
TUMOR CELLS
VERTEBRATES