Characterization of erythropoietin receptors

Conference · Fri May 01 04:00:00 EDT 1987 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6074082

Dong, Y J; Kung, C K.H.; Goldwasser, E

Erythropoietin (epo) affects erythroid progenitor cells by interacting with receptors on the cell. In this study, they have tried to define and identify epo receptors using a binding assay and by covalent cross-linking methods in purified spleen cells of mice infected with the anemia strain of the Friend virus (FVA cells) or, hemolyzed with phenylhydrazine (PHZ cells). Using asialo epo, which has greater activity in vitro than native epo, they found two populations of epo receptors on FVA cells: one with high affinity (K/sub D/ 60-70pM) and about 140-180 molecules per cell. The other with lower affinity (K/sub D/ 1-3nM) and about 550-1060 per cell. These results are similar to those reported for native epo. Further investigations were carried out by cross-linking either /sup 125/I epo or /sup 125/I asialo-epo with the intact cell. Two species with apparent molecular weights of 88 K and 105 K Daltons, after substracting epo or asialo epo molecular weight, were found in both FVA cells and PHZ cells. The binding of /sup 125/I epo and /sup 125/I asialo-epo to both kinds of receptors was competed for by unlabelled epo. In nonreducing gels there also seem to be two species: at M/sub r/ = 200 K and M/sub r/ = 280 K. Mouse thymus cell controls showed no specific binding of epo.

• Research Organization: Univ. of Chicago, IL
• OSTI ID: 6074082
• Report Number(s): CONF-870644-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 46:6
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOASSAY
CHEMICAL COMPOSITION
CHEMICAL REACTIONS
CROSS-LINKING
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
ERYTHROPOIETIN
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MEMBRANE PROTEINS
MICE
MITOGENS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
POLYMERIZATION
PROTEINS
RADIOISOTOPES
RECEPTORS
RODENTS
SOMATIC CELLS
SPLEEN CELLS
TRACER TECHNIQUES
VERTEBRATES