Use of limited protocols to evaluate the genotoxicity of hazardous wastes in mammalian cell assays: comparison to Salmonella

Journal Article · Wed Dec 31 23:00:00 EST 1986 · J. Toxicol. Environ. Health; (United States)

DOI:https://doi.org/10.1080/15287398709531065· OSTI ID:5820951

DeMarini, D M; Brusick, D J; Lewtas, J

Dichloromethane extracts of four diverse hazardous wastes (coke plant, herbicide manufacturing, pulp and paper, and oil refining) were evaluated for mutagenicity in strains TA98 and TA100 of Salmonella. These extracts also were tested for biological activity in short-term mammalian cell assays, including mutagenicity in L5178Y/TK +/- mouse lymphoma cells, chromosomal aberrations and sister chromatid exchanges in Chinese hamster ovary (CHO) cells, morphological transformation in BALB/c-3T3 cells, and teratogenic potential in mouse limb bud cells. The mammalian cell assays were performed using limited protocols that consisted of a preliminary testing of the extracts for cytotoxicity in CHO cells in order to estimate the appropriate dose range for the other assays. These assays were then performed once with only a few doses of extract; all but the mouse limb bud assay were performed in the presence of metabolic activation. Although all four of the wastes were presumptively positive for either mutation or cytogenetic effects, none of the wastes transformed BALB/c-3T3 cells. Further studies are needed to establish which mammalian cell assays, if any, might be useful complements to the Salmonella assay for the purpose of screening hazardous wastes.

• Research Organization: Environmental Protection Agency, Research Triangle Park, NC
• OSTI ID: 5820951
• Journal Information: J. Toxicol. Environ. Health; (United States), Journal Name: J. Toxicol. Environ. Health; (United States) Vol. 22:2; ISSN JTEHD
• Country of Publication: United States
• Language: English

Related Subjects

01 COAL, LIGNITE, AND PEAT
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& Peat-- Health & Safety
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BACTERIA
CARBONACEOUS MATERIALS
CELL TRANSFORMATIONS
CHO CELLS
CHROMOSOMAL ABERRATIONS
COAL
COMPARATIVE EVALUATIONS
ENERGY SOURCES
FOSSIL FUELS
FUELS
HAZARDOUS MATERIALS
HERBICIDES
MAMMALS
MATERIALS
METABOLIC ACTIVATION
MICE
MICROORGANISMS
MUTAGEN SCREENING
MUTATIONS
PAPER
PESTICIDES
PETROLEUM
RODENTS
SALMONELLA
SCREENING
SISTER CHROMATID EXCHANGES
TERATOGEN SCREENING
TERATOGENS
TOXICITY
TUMOR CELLS
VERTEBRATES