Assessment of hazardous wastes for genotoxicity

Technical Report · Tue Sep 01 04:00:00 EDT 1987

OSTI ID:5666560

DeMarini, D M; Houk, V S

The authors have evaluated a group of short-term bioassays to identify those that may be suitable for screening large numbers of diverse hazardous industrial wastes for genotoxicity. Fifteen wastes (and dichloromethane extracts of these wastes) from a variety of manufacturing processes were tested for mutagenicity in Salmonella typhimurium strains TA98 and TA100 with and without Aroclor 1254-induced rat-liver S9. Ten of these wastes were fed by gavage to F-344 male rats, and the raw urines were assayed for mutagenicity in the presence of beta-glucuronidase in strain TA98 with S9. Six of these urines were extracted by C18/methanol elution, incubated with beta-glucuronidase, and evaluated in strain TA98 with S9 and beta-glucuronidase. Fourteen of the wastes were examined for their ability to induce prophage lambda in Escherichia coli in a microsuspension assay. A second set of wastes, consisting of four industrial wastes, were evaluated in Salmonella and in a series of mammalian cell assays to measure mutagenicity, cytogenetic effects, and transformation.

• Research Organization: Environmental Protection Agency, Research Triangle Park, NC (USA). Health Effects Research Lab.
• OSTI ID: 5666560
• Report Number(s): PB-88-106984/XAB; EPA-600/D-87/285
• Country of Publication: United States
• Language: English

Related Subjects

550400 -- Genetics
550900* -- Pathology
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BIOASSAY
HAZARDOUS MATERIALS
INDUSTRIAL WASTES
MATERIALS
MICROORGANISMS
MUTAGEN SCREENING
MUTAGENESIS
SALMONELLA
SALMONELLA TYPHIMURIUM
SCREENING
TOXIC MATERIALS
TOXICITY
WASTES