New techniques for growing anaerobic bacteria: Experiments with Clostridium butyricum and Clostridium acetobutylicum

Conference · Sat Jan 01 04:00:00 EST 1983

OSTI ID:5817028

Adler, H I; Crow, W D; Hadden, C T; Hall, J; Machanoff, R

Stable membrane fragments derived from Escherichia coli produce and maintain strict anaerobic conditions when added to liquid or solid bacteriological media. Techniques for growing Clostridium butyricum and Clostridium acetobutylicum in membrane containing media are described. Liquid cultures initiated by very small inocula can be grown in direct contact with air. In solid media, colonies develop rapidly from individual cells even without incubation in anaerobic jars or similar devices. Observations on growth rates, spontaneous mutations, radiation and oxygen sensitivity of anaerobic bacteria have been made using these new techniques.

• Research Organization: Oak Ridge National Lab., TN (USA); Tennessee Univ., Oak Ridge (USA). School of Biomedical Sciences
• DOE Contract Number: AS05-76EV04568; W-7405-ENG-26
• OSTI ID: 5817028
• Report Number(s): DOE/EV/04568-38; CONF-830567-5; ORO-4568-38; ON: DE83015027
• Country of Publication: United States
• Language: English

Related Subjects

550700* -- Microbiology
560131 -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANAEROBIC CONDITIONS
BACTERIA
CELL CONSTITUENTS
CELL CULTURES
CELL MEMBRANES
CLOSTRIDIUM
CLOSTRIDIUM ACETOBUTYLICUM
CLOSTRIDIUM BUTYRICUM
CULTIVATION TECHNIQUES
ELECTROMAGNETIC RADIATION
ESCHERICHIA COLI
MEMBRANES
METHANOGENIC BACTERIA
MICROORGANISMS
MUTANTS
RADIATIONS
RADIOSENSITIVITY
SURVIVAL CURVES
ULTRAVIOLET RADIATION