Novel approach to the growth of anaerobic microorganisms

Journal Article · Thu Jan 01 04:00:00 EST 1981 · Biotechnol. Bioeng. Symp.; (United States)

OSTI ID:5297983

Adler, H I; Crow, W D

Sterile suspensions of membrane fragments derived from Escherichia coli remove oxygen rapidly and efficiently from many bacteriological media. The membranes are stable, nontoxic, and active in both solid and liquid media. Oxygen removal by the membrane fractions is a result of the presence off a cytochrome-based electron transport system that transfers hydrogen from suitable donors in the bacteriological media to oxygen, producing water as an end product. Using membrane-supplemented media, we have grown 18 species of anaerobic bacteria. For some of these organisms, membrane-containing media allow more rapid and luxuriant growth than can be achieved in media supplemented with the more commonly used but nonspecific reducing agents such as sodium thioglycollate.

• Research Organization: Oak Ridge National Lab., TN
• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 5297983
• Journal Information: Biotechnol. Bioeng. Symp.; (United States), Journal Name: Biotechnol. Bioeng. Symp.; (United States) Vol. 11; ISSN BIBSB
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ANAEROBIC CONDITIONS
ANAEROBIC DIGESTION
BACTERIA
BIOCHEMISTRY
BIOCONVERSION
CELL CONSTITUENTS
CELL MEMBRANES
CHEMISTRY
CULTURE MEDIA
CYTOCHROMES
DIGESTION
ELEMENTS
ESCHERICHIA COLI
MANAGEMENT
MEMBRANES
MICROORGANISMS
NONMETALS
OXYGEN
PIGMENTS
PROCESSING
WASTE MANAGEMENT
WASTE PROCESSING