Novel approach to the growth of anaerobic microorganisms
Conference
·
· Biotechnol. Bioeng. Symp.; (United States)
OSTI ID:5354429
Sterile suspensions of membrane fragments derived from Escherichia coli remove oxygen rapidly and efficiently from many bacteriological media. The membranes are stable, nontoxic, and active in both solid and liquid media. Oxygen removal by the membrane fractions is a result of the presence of a cytochrome-based electron transport system that transfers hydrogen from suitable donors in the bacteriological media to oxygen, producing water as an end product. Using membrane-supplemented media, we have grown 18 species of anaerobic bacteria. For some of these organisms, membrane-containing media allow more rapid and luxuriant growth than can be achieved in media supplemented with the more commonly used but nonspecific reducing agents such as sodium thioglycollate.
- Research Organization:
- Oak Ridge National Lab., TN
- OSTI ID:
- 5354429
- Report Number(s):
- CONF-810554-
- Conference Information:
- Journal Name: Biotechnol. Bioeng. Symp.; (United States) Journal Volume: 11
- Country of Publication:
- United States
- Language:
- English
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