Skip to main content
U.S. Department of Energy
Office of Scientific and Technical Information

Cell cycle-dependent regulation of excision repair of UV damage

Journal Article · · Exp. Cell Res.; (United States)
The incinsion step of excision repair of UV-damaged DNA, represented by the accumulation of DNA strand breaks in the presence of hydroxyurea and 1-..beta..-D-arabinofuranosylcytosine, was measured in cultured cells of Microtus agrestis during quiescence and at different times after release from quiescence. The UV dose-dependent kinetics of enzymic incision change rapidly through the first cell cycle after release, with a maximum potential for enzymic incision and a minimum affinity of the incision system of damage sites occurring in late G1. The potential for incision falls to a lower level as cells approach a randomly proliferating state, whilst the affinity rises. Incision activity declines rapidly after UV irradiation, leaving many damage sites unrepaired; the enhanced survival seen in cells held in quiescence after irradiation is thus partly due to some other repair mode.
Research Organization:
Univ. of Cambridge, England
OSTI ID:
5816969
Journal Information:
Exp. Cell Res.; (United States), Journal Name: Exp. Cell Res.; (United States) Vol. 142:1; ISSN ECREA
Country of Publication:
United States
Language:
English