Repair and survival after uv in quiescent and proliferating Microtus agrestis cells: different rates of incision and different dependence on DNA precursor supply
Journal Article
·
· J. Cell. Physiol.; (United States)
Cultured cells of Microtus agrestis, the common field vole, perform unscheduled DNA synthesis after uv irradiation. They respond to incubation with a DNA synthesis inhibitor (1-..beta..-D-arabinofuranosylcytosine) following uv in ways typical of cells capable of excision repair, with reduced survival and an accumulation of breaks in pre-existing DNA. Microtus cells irradiated with uv in a quiescent pre-S-phase state are more sensitive to uv than are proliferating cells, in terms of survival. Adding DNA precursors (deoxyribonucleosides), and - in the case of proliferating cells - growing in complete rather than dialysed serum, enhance uv survival. Quiescent cells show a higher rate of endonucleolytic incision of DNA after uv than do proliferating cells. The balance between incision (producing single-strand DNA breaks) and repair DNA synthesis (leading to rejoining of breaks) is shifted by the addition of deoxyribonucleosides, which suggests that DNA precursor supply is a rate-limiting factor in repair. The lower survival of quiescent cells (in the absence of added deoxyribonucleosides) may be due to insufficient precursor supply to meet the demands of the high incision rate.
- Research Organization:
- Cambridge Univ., England
- OSTI ID:
- 6252609
- Journal Information:
- J. Cell. Physiol.; (United States), Journal Name: J. Cell. Physiol.; (United States) Vol. 99:1; ISSN JCLLA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOSYNTHESIS
CELL CULTURES
CELL PROLIFERATION
DATA
DATA FORMS
DNA
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
GRAPHS
INFORMATION
MAMMALS
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
RODENTS
STRAND BREAKS
SURVIVAL TIME
SYNTHESIS
ULTRAVIOLET RADIATION
VERTEBRATES
VOLES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOSYNTHESIS
CELL CULTURES
CELL PROLIFERATION
DATA
DATA FORMS
DNA
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
GRAPHS
INFORMATION
MAMMALS
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
RODENTS
STRAND BREAKS
SURVIVAL TIME
SYNTHESIS
ULTRAVIOLET RADIATION
VERTEBRATES
VOLES