Cell cycle-related variations in uv damage and repair capacity in Chinese hamster (CHO-K1) cells. [69 ref]
Journal Article
·
· J. Cell. Physiol.; (United States)
Ultraviolet damage to CHO cell DNA, measured by formation of thymine-containing dimers, increases from mitosis to early S phase. Computer simulation of uv absorption by the DNA of an idealized CHO cell at different stages in the cell cycle resembles the cycle dependence of uv damage. Incision at uv damage sites, measured by the accumulation of breaks in preexisting DNA during 30 minutes' post-irradiation incubation with the DNA synthesis inhibitors 1-..beta..-D arabinofuranosylcytosine and hydroxyurea, increases from mitosis to interphase. Analysis of the dose dependence of DNA break accumulation indicates that both the affinity of the endonuclease for dimer sites and the maximum enzyme activity at saturating levels of dimers are significantly lower in mitosis than in interphase. The killing of CHO cells by uv is enhanced if repair is temporarily inhibited by ara C. The DNA gyrase inhibitor novobiocin prevents uv-induced incision.
- Research Organization:
- Univ. of Cambridge, England
- OSTI ID:
- 5006911
- Journal Information:
- J. Cell. Physiol.; (United States), Journal Name: J. Cell. Physiol.; (United States) Vol. 103:2; ISSN JCLLA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Cell cycle-dependent regulation of excision repair of UV damage
Inhibition of DNA excision repair by methotrexate in Chinese hamster ovary cells following exposure to ultraviolet irradiation or ethylmethanesulfonate
Hypersensitivity to mutation and sister-chromatid-exchange induction in CHO cell mutants defective in incising DNA containing UV lesions
Journal Article
·
Sun Oct 31 23:00:00 EST 1982
· Exp. Cell Res.; (United States)
·
OSTI ID:5816969
Inhibition of DNA excision repair by methotrexate in Chinese hamster ovary cells following exposure to ultraviolet irradiation or ethylmethanesulfonate
Journal Article
·
Wed Mar 14 23:00:00 EST 1990
· Cancer Research; (USA)
·
OSTI ID:7136621
Hypersensitivity to mutation and sister-chromatid-exchange induction in CHO cell mutants defective in incising DNA containing UV lesions
Journal Article
·
Thu Dec 31 23:00:00 EST 1981
· Somatic Cell Genet.; (United States)
·
OSTI ID:6170651
Related Subjects
550300 -- Cytology
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMIDES
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CULTURES
CELL CYCLE
COMPUTERIZED SIMULATION
DIMERS
DNA
DNA REPLICATION
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
ENZYME INHIBITORS
HYDROXY COMPOUNDS
HYDROXYUREA
IN VITRO
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
SIMULATION
SPECTRA
STRAND BREAKS
SURVIVAL CURVES
SYNCHRONOUS CULTURES
ULTRAVIOLET RADIATION
ULTRAVIOLET SPECTRA
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMIDES
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CULTURES
CELL CYCLE
COMPUTERIZED SIMULATION
DIMERS
DNA
DNA REPLICATION
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
ENZYME INHIBITORS
HYDROXY COMPOUNDS
HYDROXYUREA
IN VITRO
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
SIMULATION
SPECTRA
STRAND BREAKS
SURVIVAL CURVES
SYNCHRONOUS CULTURES
ULTRAVIOLET RADIATION
ULTRAVIOLET SPECTRA