In vivo dephosphorylation of WR-2721 monitored by 31P NMR spectroscopy

Journal Article · Tue Dec 31 23:00:00 EST 1985 · J. Pharmacol. Exp. Ther.; (United States)

OSTI ID:5810362

Knizner, S A; Jacobs, A J; Lyon, R C; Swenberg, C E

The in vivo dephosphorylation of the radioprotective agent S-2-(3-(aminopropylamino))ethylphosphorothioic acid (WR-2721) in male CD2F1 mice was measured by 31P NMR spectroscopy after i.p. injection. The disappearance of the WR-2721 phosphate NMR signal with time was concurrent with an increase and splitting of the inorganic phosphate NMR signal. The more acidic inorganic phosphate resonance is shown to be attributed to phosphate (inorganic phosphate) in the urine. Using regression first-order kinetic analysis of data, after i.p. injection of 600 mg/kg, the half-life of WR-2721 was determined to be 40.9 +/- 5.9 (S.D.) min (n = 10).

• Research Organization: Armed Forces Radiobiology Research Institute, Bethesda, MD
• OSTI ID: 5810362
• Journal Information: J. Pharmacol. Exp. Ther.; (United States), Journal Name: J. Pharmacol. Exp. Ther.; (United States) Vol. 1; ISSN JPETA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
CHEMICAL REACTIONS
DRUGS
IN VIVO
ISOTOPES
KINETICS
LIGHT NUCLEI
MAGNETIC RESONANCE
MAMMALS
MATERIALS
METABOLISM
MICE
NUCLEAR MAGNETIC RESONANCE
NUCLEI
ODD-EVEN NUCLEI
OXYGEN COMPOUNDS
PHOSPHATES
PHOSPHORUS 31
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
PHOSPHORYLATION
RADIOPROTECTIVE SUBSTANCES
REACTION KINETICS
RESONANCE
RODENTS
STABLE ISOTOPES
URINE
VERTEBRATES
WASTES