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RAD6/sup +/ gene of Saccharomyces cerevisiae codes for two mutationally separable deoxyribonucleic acid repair functions

Journal Article · · Mol. Cell. Biol.; (United States)
DOI:https://doi.org/10.1128/MCB.1.2.153· OSTI ID:5810071
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The response of two mutant alleles of the RAD6/sup +/ gene of Saccharomyces cerevisiae to the ochre translational suppressor SUQ5 was determined. Both the ultraviolet sensitivity phenotype and the deficiency in ultraviolet-induced mutagenesis phenotype of the rad6-1 allelle were suppressed in a (psi/sup +/) background. For the rad6-3 allelle, only the ultraviolet-sensitivity phenotype was suppressible in a (psi/sup +/) background. An SUQ5 rad6-3 (psi/sup +/) strain that was examined showed the normal rad6-3 deficiency in ultraviolet-induced mutagenesis. The authors propose that the RAD6/sup +/ gene is divided into two cistrons, RAD6A and RAD6B. RAD6A codes for an activity responsible for the error-prone repair of ultraviolet-induced lesions in deoxyribonucleic acid but is not involved in a cell's resistance to the lethal effects of ultraviolet light. RAD6B codes for an activity essential for error-free repair of potentially lethal mutagenic damage.

Research Organization:
Dept. of Biological Chemistry, California College of Medicine, Univ. of California, Irvine, CA 92717
OSTI ID:
5810071
Journal Information:
Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 1:2; ISSN MCEBD
Country of Publication:
United States
Language:
English

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
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CODONS
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GENETIC RADIATION EFFECTS
LETHAL DOSES
LETHAL RADIATION DOSE
MICROORGANISMS
MUTAGENS
MUTANTS
MUTATIONS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
PHENOTYPE
PHOTOREACTIVATION
PHYSIOLOGY
PLANTS
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RADIATIONS
RADIOINDUCTION
RADIOSENSITIVITY
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SACCHAROMYCES CEREVISIAE
STRUCTURAL CHEMICAL ANALYSIS
TRANSCRIPTION
ULTRAVIOLET RADIATION
YEASTS