Immunochemical probe for 8,5'-cycloadenosine-5'-monophosphate and its deoxy analog in irradiated nucleic acids

Journal Article · Sun Dec 01 04:00:00 EST 1985 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576589· OSTI ID:5804142

Fuciarelli, A F; Miller, G G; Raleigh, J A

Polyclonal antisera specific for 8,5'-cycloadenosine-5'-monophosphate (8,5'-cyclo-AMP) or its deoxy analog (8,5'-cyclo-dAMP) were elicited by immunizing rabbits with a conjugate prepared by the method of Johnston et al. (Biochemistry 22, 3453-3460 (1983)). A competitive enzyme-linked immunosorbent assay (ELISA) was developed and used to detect the formation of these products in irradiated solutions of polyadenylic acid (poly(A)) or DNA which were saturated with nitrous oxide, nitrogen, or oxygen. The 8,5'-cyclo-AMP or 8,5'-cyclo-dAMP moieties could be detected in poly(A) at 1.0 krad and in native DNA at 10 krad, respectively. The yield of 8,5'-cyclo-dAMP was found to be two to three times higher in irradiated double-stranded DNA than in single-stranded DNA. The hydroxyl radical appears to initiate 8,5'-cyclonucleotide formation in irradiated nucleic acids, as demonstrated by the inhibition of 8,5'-cyclo-AMP formation in irradiated poly(A) by dimethyl sulfoxide. However, irradiation under nitrous oxide, particularly at low doses, does not lead to the expected increases in the yield of the 8,5'-cyclonucleotide.

• Research Organization: Cross Cancer Institute, Edmonton, Alberta
• OSTI ID: 5804142
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 3; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560111* -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMP
BIOASSAY
CHALCOGENIDES
CHEMICAL RADIATION EFFECTS
CHEMISTRY
DNA
ELEMENTS
IMMUNE SERUMS
IMMUNOLOGY
NITROGEN
NITROGEN COMPOUNDS
NITROGEN OXIDES
NITROUS OXIDE
NONMETALS
NUCLEIC ACIDS
NUCLEOTIDES
ORGANIC COMPOUNDS
OXIDES
OXYGEN
OXYGEN COMPOUNDS
RADIATION CHEMISTRY
RADIATION EFFECTS