Resolution of 4-chlorobenzoate dehalogenase from Pseudomonas sp. strain CBS3 into three components
- Institut fuer Mikrobiologie der Universitaet Hohenheim, Stuttgart (West Germany)
Due to their widespread use and their recalcitrance, many chlorinated compounds are found as primary pollutants in our environment. Nevertheless, several bacteria have been isolated which possess the ability to degrade chlorinated hydrocarbons. Of special interest in these bacteria are the enzymes that catalyze the release of the halogen substituent. Extracts of Pseudomonas sp. strain CBS3 grown with 4-chlorobenzoate as sole carbon source contained an enzyme that converted 4-chlorobenzoate to 4-hydroxybenzoate. This enzyme was shown to consist of three components, all necessary for the reaction. Component I, which had a molecular weight of about 3,000, was highly unstable. Components II and III were stable proteins with molecular weights of about 86,000 and 92,000.
- OSTI ID:
- 5802937
- Journal Information:
- Applied and Environmental Microbiology; (USA), Journal Name: Applied and Environmental Microbiology; (USA) Vol. 57:1; ISSN 0099-2240; ISSN AEMID
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
AROMATICS
BACTERIA
BENZOIC ACID
BIODEGRADATION
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CHLORINATED AROMATIC HYDROCARBONS
DECOMPOSITION
ENZYME ACTIVITY
HALOGENATED AROMATIC HYDROCARBONS
METABOLISM
MICROORGANISMS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
PSEUDOMONAS