Cloning of Pseudomonas sp. strain CBS3 genes specifying dehalogenation of 4-chlorobenzoate

Journal Article · Wed Oct 01 00:00:00 EDT 1986 · J. Bacteriol.; (United States)

OSTI ID:6936087

Savard, P; Peloquin, L; Sylvestre, M

Halogenated benzoates have been used as models for the study of the biodegradation of herbicides and PCBs. The degradation of 4-chlorobenzoate (4-CBA) by Pseudomonas sp. strain CBS3 is thought to proceed first by the dehalogenation of 4-CBA to 4-hydroxybenzoate (4-HBA), which is then metabolized following the protocatechuate branch of the ..beta..-ketoadipate pathway. The cloning of the 4-CBA dehalogenation system was carried out by constructing a gene bank of Pseudomonas sp. strain CBS3 in Pseudomonas putida. Hybrid plasmid pPSA843 contains a 9.5-kilobase-pair fragment derived from the chromosome of Pseudomonas sp. strain CBS3. This plasmid confers on P. putida the ability to dehalogenate 4-CBA and grow on 4-CBA as the only source of carbon. However, pPSA843 did not complement mutants of P. putida unable to grow on 4-HBA (POB/sup -/), showing that the genes involved in the metabolism of 4-HBA were not cloned. Subcloning of Pseudomonas sp. strain CBS3 genes revealed that most of the insert is required for the dehalogenation of 4-CBA, suggesting that more than one gene product is involved in this dehalogenation.

• Research Organization: Universite du Quebec, Laval
• OSTI ID: 6936087
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 168:1; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

550500* -- Metabolism
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
AROMATICS
BACTERIA
BIODEGRADATION
BIOLOGICAL PATHWAYS
CELL CONSTITUENTS
CHEMICAL REACTIONS
CHLORINATED AROMATIC HYDROCARBONS
CLONING
DECOMPOSITION
DEHALOGENATION
DNA
GENE REGULATION
GENES
GENETIC ENGINEERING
HALOGENATED AROMATIC HYDROCARBONS
METABOLISM
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
PLASMIDS
PSEUDOMONAS
RECOMBINANT DNA