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Degradation of 4-chlorobenzoic acid by Arthrobacter sp

Journal Article · · Appl. Environ. Microbiol.; (United States)
OSTI ID:5909774
; ;
A mixed population, enriched and established in a defined medium, from a sewage sludge inoculum was capable of complete mineralization of 4-chlorobenzoate. An organism, identified as Arthrobacter sp., was isolated from the consortium and shown to be capable of utilizing 4-chlorobenzoate as the sole carbon and energy source in pure culture. This organism (strain TM-1), dehalogenated 4-chlorobenzoate as the initial step in the degradative pathway. The production, 4-hydroxybenzoate, was further metabolized via protocatechuate. The ability of strain TM-1 to degrade 4-chlorobenzoate in liquid medium at 25/sup 0/C was improved by the use of continuous culture and repeated sequential subculturing. Other chlorinated benzoates and the parent compound benzoate did not support growth of strain TM-1. An active cell extract was prepared and shown to dehalogenate 4-chloro-, 4-fluoro-, and 4-bromobenzoate. Dehalogenase activity had an optimum pH of 6.8 and an optimum temperature of 20/sup 0/C and was inhibited by dissolved oxygen and stimulated by manganese (Mn/sup 2 +/). Strain improvement resulted in an increase in the specific activity of the cell extract from 0.09 to 0.85 nmol of 4-hydroxybenzoate per min per mg of protein and a decrease in the doubling time of the organism from 50 to 1.6 h. 18 references, 3 figures, 2 tables.
Research Organization:
PHLS Centre for Applied Microbiology and Research, Porton Down, England
OSTI ID:
5909774
Journal Information:
Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 48:5; ISSN AEMID
Country of Publication:
United States
Language:
English

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Related Subjects

560302* -- Chemicals Metabolism & Toxicology-- Microorganisms-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AROMATICS
BACTERIA
BIODEGRADATION
BIOLOGICAL MODELS
BIOLOGICAL PATHWAYS
CHEMICAL REACTIONS
CHLORINATED AROMATIC HYDROCARBONS
CONTINUOUS CULTURE
DECOMPOSITION
ENZYME ACTIVITY
HALOGENATED AROMATIC HYDROCARBONS
METABOLISM
MICROORGANISMS
MINERALIZATION
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
SEWAGE
SEWAGE SLUDGE
SLUDGES
WASTES