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Repair of neocarzinostatin-induced deoxyribonucleic acid damage in human lymphoblastoid cells: possible involvement of apurinic/apyrimidinic sites as intermediates

Journal Article · · Biochemistry; (United States)
DOI:https://doi.org/10.1021/bi00562a008· OSTI ID:5802016
; ; ;
Neocarzinostatin (NCS) induces repair in a xeroderma pigmentosum lymphoblastoid line deficient in the ability to repair DNA damage induced with (acetoxyacetylamino)fluorene. Repair was demonstrated by the induction of repair synthesis and by the disappearance of NCS-induced single-strand breaks and/or alkaline-labile sites in DNA. Estimation of NCS-induced repair patch size indicated that only a few nucleotides were inserted per repaired region. NCS-treated bacteriophage T7 DNA requires incubation with alkaline phosphatase to make it a substrate for DNA polymerase I. NCS-reacted T7 DNA, even after phosphatase treatment, is not a substrate for a DNA polymerase ..cap alpha.. obtained from human lymphoma cells. NCS-treated T7 DNA did serve as a substrate for the DNA polymerase ..cap alpha.. when incubated with an apurinic/apyrimidinic (AP) endonuclease with associated 5'-3'-exonuclease activity. The results suggest that NCS-induced AP sites could be intermediates for the in vivo repair synthesis.
DOE Contract Number:
EY-76-S-02-2040
OSTI ID:
5802016
Journal Information:
Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 19; ISSN BICHA
Country of Publication:
United States
Language:
English

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Related Subjects

550200* -- Biochemistry
550600 -- Medicine
550900 -- Pathology
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMAL CELLS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMETABOLITES
ANTINEOPLASTIC DRUGS
AZINES
BACTERIOPHAGES
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BROMOURACILS
BUDR
DNA
DNA REPLICATION
DRUGS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
MICROORGANISMS
MOLECULAR BIOLOGY
NEOCARCINOSTATIN
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASITES
PURINES
PYRIMIDINES
RADIOMIMETIC DRUGS
RECOVERY
REPAIR
RIBOSIDES
STRAND BREAKS
URACILS
VIRUSES
XP CELLS