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Apurinic/apyrimidinic endonuclease sensitive sites as intermediates in the in vitro degradation of deoxyribonucleic acid by neocarzinostatin

Journal Article · · Biochemistry; (United States)
DOI:https://doi.org/10.1021/bi00562a007· OSTI ID:5575335
; ;
Neocarzinostatin (NCS) induces alkali-labile sites in DNA which are stabilized by NaBH/sub 4/ reduction. The stabilized sites are sensitive to an AP endonuclease from human lymphoma cells. NCS-induced degradation of supercoiled Col E1 DNA proceeds in stepwise fashion with apurinic/apyrimidinic (AP) sites as intermediates. Degradation is increased when reaction occurs in the presence of AP endonuclease, and DNA reacted with NCS can be shown to have numerous AP endonuclease sensitive sites.
Research Organization:
Univ. of Chicago, IL
OSTI ID:
5575335
Journal Information:
Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 21; ISSN BICHA
Country of Publication:
United States
Language:
English

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Related Subjects

550200* -- Biochemistry
550600 -- Medicine
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMAL CELLS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTINEOPLASTIC DRUGS
BACTERIOPHAGES
BIOLOGICAL EFFECTS
CHEMICAL REACTIONS
DECOMPOSITION
DISEASES
DNA
DRUGS
ENZYMES
ESTERASES
HYDROLASES
IN VITRO
LYMPHOMAS
MICROORGANISMS
NEOCARCINOSTATIN
NEOPLASMS
NUCLEASES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PHOSPHODIESTERASES
RADIOMIMETIC DRUGS
STRAND BREAKS
TUMOR CELLS
VIRUSES