Repair of depurinated DNA in vitro by enzymes purified from human lymphoblasts

Journal Article · Wed Feb 01 04:00:00 EST 1978 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.75.2.794· OSTI ID:6753383

Bose, K; Karran, P; Strauss, B

Alkali-labile lesions introduced into T7 DNA by treatment with methyl methanesulfonate were removed and the DNA was repaired by incubation with DNA polymerase ..cap alpha.. and nuclease from a human lymphoblastoid line followed by the addition of DNA ligase. The nuclease preparation contains both apurinic endonuclease and 5'-3' exonuclease activities. Dinucleotides appear to be the first product of exonuclease action. Repair of methyl methanesulfonate-induced damage can occur by the insertion of only a few nucleotides per lesion as in vivo.

• Research Organization: Univ. of Chicago
• OSTI ID: 6753383
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 75:2; ISSN PNASA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BACTERIOPHAGES
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CULTURES
DNA
ENZYMES
IN VITRO
LYMPHATIC SYSTEM
MAMMALS
MAN
MICROORGANISMS
NUCLEASES
NUCLEIC ACIDS
NUCLEOTIDES
ORGANIC COMPOUNDS
PARASITES
PHOSPHOTRANSFERASES
POLYMERASES
PRIMATES
RECOVERY
REPAIR
TRANSFERASES
VERTEBRATES
VIRUSES