Interleukin 1 beta initially sensitizes and subsequently protects murine intestinal stem cells exposed to photon radiation

Journal Article · Wed May 01 04:00:00 EDT 1991 · Cancer Research; (USA)

OSTI ID:5799974

Hancock, S L; Chung, R T; Cox, R S; Kallman, R F ^[1]

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1. Stanford Univ. School of Medicine, CA (USA)

Interleukin 1 (IL-1) has been shown to prevent early bone marrow-related death following total-body irradiation, by protecting hematopoietic stem cells and speeding marrow repopulation. This study assesses the effect of IL-1 on the radiation response of the intestinal mucosal stem cell, a nonhematopoietic normal cell relevant to clinical radiation therapy. As observed with bone marrow, administration of human recombinant IL-1 beta (4 micrograms/kg) to C3H/Km mice 20 h prior to total-body irradiation modestly protected duodenal crypt cells. In contrast to bone marrow, IL-1 given 4 or 8 h before radiation sensitized intestinal crypt cells. IL-1 exposure did not substantially alter the slope of the crypt cell survival curve but did affect the shoulder: the X-ray survival curve was offset to the right by 1.01 +/- 0.06 Gy when IL-1 was given 20 h earlier and by 1.28 +/- 0.08 Gy to the left at the 4-h interval. Protection was greatest when IL-1 was administered 20 h before irradiation, but minimal effects persisted as long as 7 days after a single injection. The magnitude of radioprotection at 20 h or of radiosensitization at 4 h increased rapidly as IL-1 dose increased from 0 to 4 micrograms/kg. However, doses ranging from 10 to 100 micrograms/kg produced no further difference in radiation response. Animals treated with saline or IL-1 had similar core temperatures from 4 to 24 h after administration, suggesting that thermal changes were not responsible for either sensitization or protection. Mice irradiated 20 h after IL-1 had significantly greater crypt cell survival than saline-treated irradiated controls at all assay times, which ranged from 54 to 126 h following irradiation. The intervals to maximum crypt depopulation and initiation of repopulation were identical in both saline- and IL-1-treated groups.

• OSTI ID: 5799974
• Journal Information: Cancer Research; (USA), Journal Name: Cancer Research; (USA) Vol. 51:9; ISSN CNREA; ISSN 0008-5472
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
DIGESTIVE SYSTEM
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
GASTROINTESTINAL TRACT
GROWTH FACTORS
INTESTINES
IONIZING RADIATIONS
LYMPHOKINES
MAMMALS
MEMBRANES
MICE
MITOGENS
MUCOUS MEMBRANES
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIATION EFFECTS
RADIATION PROTECTION
RADIATIONS
RADIOSENSITIVITY EFFECTS
RODENTS
SOMATIC CELLS
STEM CELLS
VERTEBRATES
X RADIATION