Stem cell factor enhances the survival of murine intestinal stem cells after photon irradiation

Journal Article · Sat Apr 01 04:00:00 EST 1995 · Radiation Research

DOI:https://doi.org/10.2307/3578961· OSTI ID:86328

Leigh, B R; Khan, W; Hancock, S L ^[1]

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1. Stanford Univ. Hospital, Stanford, CA (United States); and others

Recombinant rat stem cell factor (SCF) has been shown to decrease lethality in mice exposed to total-body irradiation (TBI) in the lower range of lethality through radioprotection of hematopoietic stem cells and acceleration of bone marrow repopulation. This study evaluates the effect of SCF on the survival of the intestinal mucosal stem cell after TBI. This non-hematopoietic cell is clinically relevant. Gastrointestinal toxicity is common during and after abdominal and pelvic radiation therapy and limits the radiation dose in these regions. As observed with bone marrow, the administration of SCF to mice prior to TBI enhanced the survival of mouse duodenal crypt stem cells. The maximum enhancement of survival was seen when 100 {mu}/kg of SCF was given intraperitoneally 8 h before irradiation. This regimen increased the survival of duodenal crypt stem cells after 12.0 Gy TBI from 22.5 {+-} 0.7 per duodenal cross section for controls to 30.0 {+-} 1.7 after treatment with SCF (P=0.03). The TBI dose producing 50% mortality of 6 days (LD{sub 50/6}) was increased from 14.9 Gy for control mice to 19.0 Gy for mice treated with SCF (dose modification factor = 1.28). These findings demonstrate that SCF (dose modification factor = 1.28). These findings demonstrate that SCF has radioprotective effects on a non-hematopoietic stem cell population and suggest that SCF may be of clinical value in preventing radiation injury to the intestine. 29 refs., 4 figs.

• Sponsoring Organization: USDOE
• OSTI ID: 86328
• Journal Information: Radiation Research, Journal Name: Radiation Research Journal Issue: 1 Vol. 142; ISSN 0033-7587; ISSN RAREAE
• Country of Publication: United States
• Language: English

Related Subjects

56 BIOLOGY AND MEDICINE
APPLIED STUDIES
CRYPT CELLS
FEASIBILITY STUDIES
GASTROINTESTINAL TRACT
MICE
MORTALITY
RADIATION SYNDROME
RADIOPROTECTIVE SUBSTANCES
STEM CELLS
SURVIVAL CURVES
WHOLE-BODY IRRADIATION