Spontaneous and mitomycin-C-induced micronuclei in human lymphocytes exposed to extremely low frequency pulsed magnetic fields

Journal Article · Mon Apr 15 04:00:00 EDT 1991 · Biochemical and Biophysical Research Communications; (USA)

DOI:https://doi.org/10.1016/0006-291X(91)90908-P· OSTI ID:5798878

Scarfi, M R; Bersani, F; Cossarizza, A; Monti, D; Castellani, G; Cadossi, R; Franceschetti, G; Franceschi, C ^[1]

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1. Istituto di Ricerca per l'Elettromagnetismo e i Componenti Elettronici Consiglio Nazionale delle Ricerche, Napoli (Italy)

The cytokinesis block micronucleus method, a very sensitive cytogenetic assay, was used to ascertain the possible genotoxic effects of extremely low frequency pulsed magnetic fields in phytohemagglutinin-stimulated human lymphocytes cultures from 16 healthy donors. Four conditions were studied: lymphocytes not exposed to the field (control cultures); lymphocytes exposed to the field; lymphocytes treated with mitomycin-C and not exposed to the field; and lymphocytes treated with mitomycin-C and exposed to the field. Mitomycin-C-treated cultures were used as control for the micronucleus method, because it is known that mitomycin-C is a potent genotoxic agent, capable of inducing micronuclei. The frequency of micronuclei in field-exposed cultures was similar to the spontaneous frequency observed in control unexposed-cultures. Moreover, the exposure to pulsed magnetic fields did not affect the frequency of micronuclei induced by mitomycin-C, suggesting that, in the experimental conditions used, this kind of field neither affected the integrity of chromosomes nor interfered with the genotoxic activity of mitomycin-C.

• OSTI ID: 5798878
• Journal Information: Biochemical and Biophysical Research Communications; (USA), Journal Name: Biochemical and Biophysical Research Communications; (USA) Vol. 176:1; ISSN BBRCA; ISSN 0006-291X
• Country of Publication: United States
• Language: English

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