Conversions of excision-repairable DNA lesions to micronuclei within one cell cycle in human lymphocytes

Journal Article · Wed Jan 01 04:00:00 EST 1992 · Environmental and Molecular Mutagenesis; (United States)

DOI:https://doi.org/10.1002/em.2850190106· OSTI ID:5481736

Fenech, M; Neville, S ^[1]

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1. CSIRO, Adelaide (Australia)

The human lymphocyte micronucleus (MN) assay is relatively insensitive to genotoxic agents that predominantly induce excision-repairable lesions such as adducts and abasic sites. In this study the authors have explored the possibility of using cytosine arabinoside (ARA) to convert excision-repairable DNA lesions to micronuclei (MN) within one cell cycle. The system consisted of human lymphocytes as target cells, the cytokinesis-block (CB) method for identifying cells that had completed one nuclear division only, and X-rays, methylnitrosourea (MNU), and ultraviolet light (UV) as mutagens. With each mutagen they have observed significant increments induced MN in the cultures that had also been treated with ARA during G{sub 1}. These observations suggested that the combined ARA and cytokinesis-block micronucleus (CBMN) method may enhance the detection of exposure to genotoxic agents that predominantly induce excision-repairable lesions.

• OSTI ID: 5481736
• Journal Information: Environmental and Molecular Mutagenesis; (United States), Journal Name: Environmental and Molecular Mutagenesis; (United States) Vol. 19:1; ISSN EMMUE; ISSN 0893-6692
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALDEHYDES
AMINES
ANIMAL CELLS
ANIMALS
ARABINOSE
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CARBONIC ACID DERIVATIVES
CELL CONSTITUENTS
CELL NUCLEI
CONNECTIVE TISSUE CELLS
CYTOSINE
DNA
DNA REPAIR
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
EXTREME ULTRAVIOLET RADIATION
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
HETEROCYCLIC COMPOUNDS
IONIZING RADIATIONS
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MAN
MATERIALS
METHYL NITROSOUREA
MONOSACCHARIDES
MUTAGENS
NITROSO COMPOUNDS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PENTOSES
PRIMATES
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
SACCHARIDES
SOMATIC CELLS
ULTRAVIOLET RADIATION
VERTEBRATES
X RADIATION